Vázquez-Memije M E, Izquierdo-Reyes V, Delhumeau-Ongay G
Sección de Regulación Metabólica, Unidad de Investigación Biomédica del Centro Médico Nacional, México.
Arch Biochem Biophys. 1988 Jan;260(1):67-74. doi: 10.1016/0003-9861(88)90425-0.
Albumin-free testis mitochondrial ATPase activity failed to be stimulated by either 2,4-dinitrophenol (DNP) or carbonyl cyanide rho-trifluoromethoxyphenylhydrazone (FCCP). DNP scarcely enhanced the state 4 respiration and mitochondria proved to be poorly coupled. When 1% bovine serum albumin was added to the isolation medium, DNP or FCCP stimulated ATPase nearly twofold and the dose-response curves for the uncouplers on the QO2 reached a plateau at five- to sixfold. The DNP coupling index (q) also showed a 30-40% improvement. A dose-response curve for oligomycin on the rate of [gamma-32P]ATP synthesis showed a stimulation of ATP synthase activity by 10-100 ng inhibitor/mg protein, suggesting a possible blockade of "open" F0 channels. In the albumin preparation oligomycin inhibited ATP synthesis in the range 10-100 ng/mg protein. Since testis ATPase is known to be loosely bound to the membrane, an effect of albumin, improving tightness in the interaction of the F1 and the F0 sectors of the ATPase, is suggested.
无白蛋白的睾丸线粒体ATP酶活性不受2,4-二硝基苯酚（DNP）或羰基氰化物间三氟甲氧基苯腙（FCCP）的刺激。DNP几乎不增强状态4呼吸，且线粒体被证明偶联性较差。当向分离培养基中添加1%牛血清白蛋白时，DNP或FCCP可使ATP酶活性提高近两倍，解偶联剂对QO2的剂量反应曲线在五到六倍时达到平台期。DNP偶联指数（q）也显示提高了30 - 40%。寡霉素对[γ-32P]ATP合成速率的剂量反应曲线显示，10 - 100 ng抑制剂/ mg蛋白质可刺激ATP合酶活性，提示可能阻断了“开放”的F0通道。在白蛋白制剂中，寡霉素在10 - 100 ng/ mg蛋白质范围内抑制ATP合成。由于已知睾丸ATP酶与膜松散结合，提示白蛋白具有改善ATP酶F1和F0部分相互作用紧密性的作用。
