通过 CRISPR/Cas 系统的偏移切口实现高效的基因组修饰小鼠的产生。
Efficient generation of genome-modified mice via offset-nicking by CRISPR/Cas system.
机构信息
Department of Animal Resource Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1, Tokyo 113-8657, Japan.
Department of Animal Resource Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1, Tokyo 113-8657, Japan.
出版信息
Biochem Biophys Res Commun. 2014 Mar 21;445(4):791-4. doi: 10.1016/j.bbrc.2014.01.141. Epub 2014 Jan 31.
The mammalian zygote-mediated CRISPR/Cas system can efficiently generate targeted genome-modified animals. However, this system is limited by the risk of off-target mutations. Here we show that offset-nicking by Cas9 nickase and paired gRNAs allows us to generate region deleted mice and targeted knock-in mice without off-target mutations.
哺乳动物受精卵介导的 CRISPR/Cas 系统可以有效地产生靶向基因组修饰的动物。然而，该系统受到脱靶突变的风险限制。在这里，我们展示了 Cas9 核酸酶和配对 gRNA 的偏移切割允许我们在不产生脱靶突变的情况下生成缺失区域的小鼠和靶向敲入小鼠。
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