Department of Biology and Ecology, Faculty of Science, University of Ostrava, Ostrava, Czech Republic; Department of Genetics, Faculty of Natural Sciences, Comenius University, Bratislava, Slovakia.
Department of Genetics, Faculty of Natural Sciences, Comenius University, Bratislava, Slovakia.
FEBS Lett. 2014 Mar 3;588(5):783-8. doi: 10.1016/j.febslet.2014.01.034. Epub 2014 Feb 1.
Euglena gracilis possesses secondary plastids of green algal origin. In this study, E. gracilis expressed sequence tags (ESTs) derived from polyA-selected mRNA were searched and several ESTs corresponding to plastid genes were found. PCR experiments failed to detect SL sequence at the 5'-end of any of these transcripts, suggesting plastid origin of these polyadenylated molecules. Quantitative PCR experiments confirmed that polyadenylation of transcripts occurs in the Euglena plastids. Such transcripts have been previously observed in primary plastids of plants and algae as low-abundance intermediates of transcript degradation. Our results suggest that a similar mechanism exists in secondary plastids.
衣藻具有绿藻来源的次生质体。在这项研究中，我们搜索了衣藻多聚 A 选择的 mRNA 衍生的表达序列标签 (ESTs)，并找到了几个对应质体基因的 EST。PCR 实验未能检测到这些转录本 5'端的 SL 序列，表明这些多聚腺苷酸化分子具有质体来源。定量 PCR 实验证实，转录本在衣藻质体中发生多聚腺苷酸化。这些转录本以前在植物和藻类的初级质体中作为转录本降解的低丰度中间产物被观察到。我们的结果表明，类似的机制存在于次生质体中。
