Faculty of Pharmacy and Faculty of Medicine, University of Tripoli, Tripoli, Libya.
Am J Trop Med Hyg. 2014 Apr;90(4):724-7. doi: 10.4269/ajtmh.13-0715. Epub 2014 Feb 3.
Methicillin-resistant Staphylococcus aureus (MRSA) colonized children are at an increased risk of developing infections than methicillin-sensitive S. aureus colonized children. Nasal specimens from inpatient children, mothers of inpatient children, healthcare workers, and outpatient children at Tripoli Children Hospital (TCH) were examined for MRSA by chromogenic MRSA ID medium. Susceptibility of MRSA isolates to antibiotics was determined by the disc diffusion method. The nasal carriage rate of MRSA among inpatient children (8.3%, 24 of 289), their mothers (11%, 22 of 200), and healthcare workers (12.4%, 22 of 178) was significantly higher than among outpatient children (2.2%, 2 of 91) (P < 0.05, P < 0.02, and P < 0.006, respectively). Of the examined MRSA isolates (N = 35) 10 (28.6%) were positive for Panton-Valentine leucocidin genes by polymerase chain reaction. Multidrug resistance was found in 24.3% (17 of 70) of MRSA isolates. Nasal carriage of multidrug-resistant Panton-Valentine leucocidin-positive MRSA is not uncommon among inpatient children and their mothers in Tripoli.
耐甲氧西林金黄色葡萄球菌（MRSA）定植的儿童比甲氧西林敏感金黄色葡萄球菌定植的儿童发生感染的风险更高。通过显色性耐甲氧西林金黄色葡萄球菌 ID 培养基，对来自的黎波里儿童医院（TCH）住院儿童、住院儿童的母亲、医护人员和门诊儿童的鼻腔标本进行 MRSA 检测。通过纸片扩散法测定 MRSA 分离株对抗生素的敏感性。住院儿童（8.3%，24/289）、其母亲（11%，22/200）和医护人员（12.4%，22/178）中 MRSA 的鼻腔携带率明显高于门诊儿童（2.2%，2/91）（P<0.05、P<0.02 和 P<0.006）。在检测的 35 株 MRSA 分离株中，有 10 株（28.6%）通过聚合酶链反应对 Panton-Valentine 白细胞素基因呈阳性。24.3%（17/70）的 MRSA 分离株存在多药耐药性。耐多药、携带 Panton-Valentine 白细胞素阳性 MRSA 的儿童在的黎波里住院儿童及其母亲中并不少见。
