大鼠脑皮质和海马核及突触体组分中主要阳离子对针对焦谷氨酸6[¹²⁵I-酪氨酸8]SP6-11的肽酶活性的影响。

The effects of the main body cations on the peptidase(s) activity against pyroGlu6[125I-Tyr8]SP6-11 in the nuclear and synaptosomal fraction of the cortex and hippocampus of rat brain.

Turski W A, Lachowicz L

Department of Biochemistry II, School of Medicine, Lódź, Poland.

Int J Biochem. 1987;19(12):1233-6. doi: 10.1016/0020-711x(87)90109-1.

Abstract

Peptidase(s) activity of nuclear and synaptosomal fraction from cortex and hippocampus of rat brain against pyroGlu6[125I-Tyr8]SP6-11 was evaluated in different concentration of Ca2+, Mg2+, K+ and Na+ in about "isotonic" conditions. 2. The effects of studied ions on the peptidase activities forming N-terminal and C-terminal fragments are different especially in synaptosomes of both areas. 3. The differences of ionic requirements for N- and C-forming activities are particularly relevant for Ca2+ at the cortex and K+ at the hippocampus. 4. Ca2+ activate forming of N-terminal fragments in the nuclear fraction whereas inhibit it in synaptosomes from both areas. 5. The ionic requirements for C-terminal fragments' formation in synaptosomes of both areas are contradictory.

摘要

在大约“等渗”条件下，于不同浓度的Ca2+、Mg2+、K+和Na+中，评估大鼠大脑皮层和海马体的细胞核及突触体部分针对焦谷氨酸6[125I-酪氨酸8]速激肽6-11的肽酶活性。2. 所研究的离子对形成N端和C端片段的肽酶活性的影响不同，尤其是在这两个区域的突触体中。3. 对于N端和C端形成活性的离子需求差异，在皮层中Ca2+尤为显著，在海马体中K+尤为显著。4. Ca2+激活细胞核部分N端片段的形成，而在两个区域的突触体中则抑制其形成。5. 两个区域突触体中C端片段形成的离子需求相互矛盾。