Liu Qing-Shan, Chen Xiao-Yu, Zhuang Shu-Juan, Li Ke-Qin
Minzu University of China, State Nationalities Affairs Commission and Department of Educational Key Lab of Minority Traditional Medicine, Beijing 100081, China.
Zhongguo Zhong Yao Za Zhi. 2013 Nov;38(21):3776-81.
The neural stem cells (NSCs), play a crucial role in stroke treatment, which can be regulated by a few of traditional Chinese medicines. In this study, the effect of the Mongolian medicine Baimai powder effective compounds group (BMECG) on the proliferation of NSCs has been investigated. The cultured NSCs which were isolated from newborn rat cerebral cortical in vitro were exposed to oxygen glucose deprivation/reoxgenation (OGD/R). The CFSE immunofluorescence staining was employed to identify the proliferation of NSCs by flow cytometry. Furthermore, the bilateral carotid artery occlusion (BCAO) was established on Kunming mice, and all groups were ig for 7 d respectively. The neurobehavioral changes was studied with rota-rod treadmill test, after that, the brain of mice were detected by immunohistochemistry with labeling of Nestin and pathological observation at 7 days after BCAO. It was found that, proliferation of NSCs was increased by BMECG in in vitro and in vivo. And BMECG significantly improved the time of staying in the rota-rod, it can promote the foundction of in cerebral cortex. It is concluded that these results further support the hypothesis that neuroprotective effect of BMECG may relate to the ability of stimulating self-renew of NSCs, which can be provided a new insight and strategy of anti-neuropathy of stroke.
神经干细胞（NSCs）在中风治疗中发挥着关键作用，其可受到多种中药的调节。在本研究中，对蒙药白脉散有效成分组（BMECG）对神经干细胞增殖的影响进行了研究。将体外分离自新生大鼠大脑皮层的培养神经干细胞暴露于氧糖剥夺/复氧（OGD/R）环境。采用CFSE免疫荧光染色通过流式细胞术鉴定神经干细胞的增殖情况。此外，在昆明小鼠上建立双侧颈动脉闭塞（BCAO）模型，各实验组分别灌胃7天。采用转棒试验研究神经行为变化，之后在BCAO术后7天通过免疫组织化学检测小鼠大脑中Nestin标记情况并进行病理观察。结果发现，BMECG在体外和体内均可增加神经干细胞的增殖。并且BMECG显著延长了小鼠在转棒上停留的时间，可促进大脑皮层的修复。得出结论，这些结果进一步支持了BMECG的神经保护作用可能与刺激神经干细胞自我更新能力有关的假说，这可为中风抗神经病变提供新的见解和策略。
