a Laboratory of Bioflavors, Department of Food Science, Faculty of Food Engineering , University of Campinas - UNICAMP , Campinas , Brazil.
Crit Rev Biotechnol. 2015;35(3):313-25. doi: 10.3109/07388551.2013.855161.
Throughout human history, natural products have been the basis for the discovery and development of therapeutics, cosmetic and food compounds used in industry. Many compounds found in natural organisms are rather difficult to chemically synthesize and to extract in large amounts, and in this respect, genetic and metabolic engineering are playing an increasingly important role in the production of these compounds, such as new terpenes and terpenoids, which may potentially be used to create aromas in industry. Terpenes belong to the largest class of natural compounds, are produced by all living organisms and play a fundamental role in human nutrition, cosmetics and medicine. Recent advances in systems biology and synthetic biology are allowing us to perform metabolic engineering at the whole-cell level, thus enabling the optimal design of microorganisms for the efficient production of drugs, cosmetic and food additives. This review describes the recent advances made in the genetic and metabolic engineering of the terpenes pathway with a particular focus on systems biotechnology.
纵观人类历史，天然产物一直是发现和开发治疗药物、化妆品和食品化合物的基础，这些化合物在工业中得到应用。许多在天然生物中发现的化合物在化学合成和大量提取方面都相当困难，在这方面，遗传和代谢工程在这些化合物的生产中发挥着越来越重要的作用，例如新的萜类化合物和类萜，它们可能被用于在工业中创造香气。萜类化合物属于最大的一类天然化合物，由所有生物产生，在人类营养、化妆品和医药中起着至关重要的作用。系统生物学和合成生物学的最新进展使我们能够在全细胞水平上进行代谢工程，从而能够优化微生物的设计，以高效生产药物、化妆品和食品添加剂。本文综述了萜类化合物途径的遗传和代谢工程的最新进展，特别关注系统生物技术。
