Liu Shun-Cheng, Xu Longxing, Sun Yuejia, Yuan Lijie, Xu Hong, Song Xiaoming, Sun Liangdan
Hebei Key Laboratory for Chronic Diseases, Tangshan Key Laboratory for Preclinical and Basic Research on Chronic Diseases, School of Basic Medical Sciences, North China University of Science and Technology, Tangshan 063210, Hebei, China.
Health Science Center, North China University of Science and Technology, Tangshan 063210, Hebei, China.
Biodes Res. 2024 Nov 12;6:0051. doi: 10.34133/bdr.0051. eCollection 2024.
Terpenes are natural secondary metabolites with isoprene as the basic structural unit; they are widely found in nature and have potential applications as advanced fuels, pharmaceutical ingredients, and agricultural chemicals. However, traditional methods are inefficient for obtaining terpenes because of complex processes, low yields, and environmental unfriendliness. The unconventional oleaginous yeast , with a clear genetic background and complete gene editing tools, has attracted increasing attention for terpenoid synthesis. Here, we review the synthetic biology tools for , including promoters, terminators, selection markers, and autonomously replicating sequences. The progress and emerging trends in the metabolic engineering of for terpenoid synthesis are further summarized. Finally, potential future research directions are envisioned.
萜类化合物是以异戊二烯为基本结构单元的天然次生代谢产物；它们在自然界中广泛存在，作为先进燃料、药物成分和农用化学品具有潜在应用价值。然而，传统方法获取萜类化合物效率低下，原因在于工艺复杂、产量低且不环保。具有清晰遗传背景和完整基因编辑工具的非常规产油酵母，在萜类化合物合成方面越来越受到关注。在此，我们综述了用于（此处原文缺失具体对象）的合成生物学工具，包括启动子、终止子、选择标记和自主复制序列。进一步总结了（此处原文缺失具体对象）用于萜类化合物合成的代谢工程的进展和新趋势。最后，展望了未来潜在的研究方向。
