Lewis C G, Fields M, Craft N, Yang C Y, Reiser S
Carbohydrate Nutrition Laboratory, U.S. Department of Agriculture, Beltsville, Maryland 20705.
J Am Coll Nutr. 1988 Feb;7(1):27-34. doi: 10.1080/07315724.1988.10720217.
This study describes the progressive changes in exocrine pancreatic enzyme specific activities from rats provided with a diet containing either 62% starch (S) or fructose (F) with 6.0 ppm copper (+Cu) or 0.6 ppm (-Cu) from 21 to 61 days of age. Copper-deficient rats had significantly reduced pancreatic weight and DNA, RNA, protein and copper content and superoxide dismutase (SOD) activity after consuming diet for 39 days. These reductions were far more severe in F-Cu than in S-Cu rats. Copper deficiency significantly reduced pancreatic amylase and lipase specific activities, but the effect was more severe in the F-Cu dietary group. Chymotrypsinogen and trypsinogen specific activities were reduced only in the F-Cu dietary group and the CHO x Cu interaction was significant. High dietary fructose and low dietary copper in combination interact to produce severe reductions in pancreatic amylase, lipase, chymotrypsinogen, and trypsinogen specific activities which could not be explained by either copper deficiency or by an increase in dietary fructose alone.
本研究描述了21至61日龄的大鼠在食用含62%淀粉（S）或果糖（F）且铜含量为6.0 ppm（+Cu）或0.6 ppm（-Cu）的饮食后，胰腺外分泌酶比活性的渐进性变化。缺铜大鼠在食用该饮食39天后，胰腺重量、DNA、RNA、蛋白质和铜含量以及超氧化物歧化酶（SOD）活性显著降低。这些降低在F-Cu大鼠中比在S-Cu大鼠中更为严重。缺铜显著降低了胰腺淀粉酶和脂肪酶的比活性，但在F-Cu饮食组中这种影响更为严重。只有在F-Cu饮食组中胰凝乳蛋白酶原和胰蛋白酶原的比活性降低，且碳水化合物与铜的相互作用显著。高果糖饮食和低铜饮食共同作用，导致胰腺淀粉酶、脂肪酶、胰凝乳蛋白酶原和胰蛋白酶原的比活性严重降低，这不能仅用缺铜或饮食中果糖增加来解释。
