Sjöstrand K, Wess G, Ljungvall I, Häggström J, Merveille A-C, Wiberg M, Gouni V, Lundgren Willesen J, Hanås S, Lequarré A-S, Mejer Sørensen L, Wolf J, Tiret L, Kierczak M, Forsberg S, McEntee K, Battaille G, Seppälä E, Lindblad-Toh K, Georges M, Lohi Hannes, Chetboul V, Fredholm M, Höglund K
Faculty of Veterinary Medicine and Animal Science, Swedish University of Agricultural Sciences, Uppsala, Sweden.
J Vet Intern Med. 2014 Mar-Apr;28(2):451-7. doi: 10.1111/jvim.12310. Epub 2014 Feb 3.
Measurement of plasma concentration of natriuretic peptides (NPs) is suggested to be of value in diagnosis of cardiac disease in dogs, but many factors other than cardiac status may influence their concentrations. Dog breed potentially is 1 such factor.
To investigate breed variation in plasma concentrations of pro-atrial natriuretic peptide 31-67 (proANP 31-67) and N-terminal B-type natriuretic peptide (NT-proBNP) in healthy dogs.
535 healthy, privately owned dogs of 9 breeds were examined at 5 centers as part of the European Union (EU) LUPA project.
Absence of cardiovascular disease or other clinically relevant organ-related or systemic disease was ensured by thorough clinical investigation. Plasma concentrations of proANP 31-67 and NT-proBNP were measured by commercially available ELISA assays.
Overall significant breed differences were found in proANP 31-67 (P < .0001) and NT-proBNP (P < .0001) concentrations. Pair-wise comparisons between breeds differed in approximately 50% of comparisons for proANP 31-67 as well as NT-proBNP concentrations, both when including all centers and within each center. Interquartile range was large for many breeds, especially for NT-proBNP. Among included breeds, Labrador Retrievers and Newfoundlands had highest median NT-proBNP concentrations with concentrations 3 times as high as those of Dachshunds. German Shepherds and Cavalier King Charles Spaniels had the highest median proANP 31-67 concentrations, twice the median concentration in Doberman Pinschers.
Considerable interbreed variation in plasma NP concentrations was found in healthy dogs. Intrabreed variation was large in several breeds, especially for NT-proBNP. Additional studies are needed to establish breed-specific reference ranges.
血浆利钠肽（NP）浓度的测定对犬类心脏病的诊断具有重要价值，但除心脏状况外，许多其他因素也可能影响其浓度。犬种可能就是其中一个因素。
研究健康犬血浆中前心钠素31 - 67（proANP 31 - 67）和N末端B型利钠肽（NT - proBNP）浓度的品种差异。
作为欧盟（EU）LUPA项目的一部分，在5个中心对9个品种的535只健康私有犬进行了检查。
通过全面的临床检查确保无心血管疾病或其他临床相关的器官相关或全身性疾病。采用市售酶联免疫吸附测定法（ELISA）检测proANP 31 - 67和NT - proBNP的血浆浓度。
在proANP 31 - 67（P <.0001）和NT - proBNP（P <.0001）浓度方面发现了总体显著的品种差异。在所有中心以及每个中心内，品种间的两两比较中，proANP 31 - 67和NT - proBNP浓度的比较约有50%存在差异。许多品种的四分位间距较大，尤其是NT - proBNP。在所纳入的品种中，拉布拉多寻回犬和纽芬兰犬的NT - proBNP浓度中位数最高，是腊肠犬的3倍。德国牧羊犬和查理士王小猎犬的proANP 31 - 67浓度中位数最高，是杜宾犬中位数浓度的两倍。
在健康犬中发现血浆NP浓度存在显著的品种间差异。几个品种的品种内差异也很大，尤其是NT - proBNP。需要进一步研究以建立特定品种的参考范围。
