脂肪间充质干细胞对慢性阻塞性肺疾病豚鼠模型氧化应激水平、肺气肿及白细胞的影响。
The effect of adipose derived stromal cells on oxidative stress level, lung emphysema and white blood cells of guinea pigs model of chronic obstructive pulmonary disease.
机构信息
Neurogenic Inflammation Research Centre and Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad 9177948564, Iran.
出版信息
Daru. 2014 Feb 4;22(1):26. doi: 10.1186/2008-2231-22-26.
BACKGROUND
Chronic obstructive pulmonary disease (COPD) is a worldwide epidemic disease and a major cause of death and disability. The present study aimed to elucidate pharmacological effects of adipose derived stromal cells (ASCs) on pathological and biochemical factors in a guinea pig model of COPD. Guinea pigs were randomized into 5 groups including: Control, COPD, COPD + intratracheal delivery of PBS as a vehicle (COPD-PBS), COPD + intratracheal delivery of ASCs (COPD-ITASC) and COPD + intravenous injection of ASCs (COPD-IVASC). COPD was induced by exposing animals to cigarette smoke for 3 months. Cell therapy was performed immediately after the end of animal exposure to cigarette smoke and 14 days after that, white blood cells, oxidative stress indices and pathological changes of the lung were measured.
RESULTS
Compared with control group, emphysema was clearly observed in the COPD and COPD-PBS groups (p < 0.001). Lung histopathologic changes of COPD-ITASC and COPD-IVASC groups showed non-significant improvement compared to COPD-PBS group. The COPD-ITASC group showed a significant increase in total WBC compared to COPD-PBS group but there was not a significant increase in this regard in COPD-IVASC group. The differential WBC showed no significant change in number of different types of leukocytes. The serum level of malondialdehyde (MDA) significantly decreased but thiol groups of broncho-alveolar lavage fluid (BALF) increased in both cell treated groups (p < 0.05 for all cases). Weight of animals decreased during smoke exposure and improved after PBS or cell therapy. However, no significant change was observed between the groups receiving PBS and the ones receiving ASCs.
CONCLUSION
Cell therapy with ASCs can help in reducing oxidative damage during smoking which may collectively hold promise in attenuation of the severity of COPD although the lung structural changes couldn't be ameliorated with these pharmacological therapeutic methods.
背景
慢性阻塞性肺疾病（COPD）是一种全球性的流行疾病，也是导致死亡和残疾的主要原因。本研究旨在阐明脂肪基质细胞（ASCs）对 COPD 豚鼠模型中病理和生化因素的药理作用。豚鼠随机分为 5 组：对照组、COPD 组、COPD 组+气管内滴注 PBS 作为载体（COPD-PBS 组）、COPD 组+气管内滴注 ASCs（COPD-ITASC 组）和 COPD 组+静脉注射 ASCs（COPD-IVASC 组）。通过使动物暴露于香烟烟雾中 3 个月来诱导 COPD。在动物暴露于香烟烟雾结束后立即进行细胞治疗，并在 14 天后测量白细胞、氧化应激指标和肺的病理变化。
结果
与对照组相比，COPD 组和 COPD-PBS 组明显观察到肺气肿（p<0.001）。COPD-ITASC 组和 COPD-IVASC 组的肺组织病理学变化与 COPD-PBS 组相比无明显改善。与 COPD-PBS 组相比，COPD-ITASC 组的总白细胞计数明显增加，但在 COPD-IVASC 组中，白细胞计数没有显著增加。不同类型白细胞的差异白细胞计数无明显变化。血清丙二醛（MDA）水平显著降低，但支气管肺泡灌洗液（BALF）的巯基基团增加，在接受细胞治疗的两组中均如此（所有情况下均为 p<0.05）。在吸烟期间动物体重下降，在接受 PBS 或细胞治疗后体重增加。然而，在接受 PBS 和接受 ASCs 的两组之间没有观察到显著变化。
结论
用 ASCs 进行细胞治疗可以帮助减少吸烟引起的氧化损伤，尽管这些药物治疗方法不能改善肺结构变化，但它们可能对减轻 COPD 的严重程度有一定作用。
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