Smith C P, Chater K F
John Innes Institute, Norwich, UK.
Mol Gen Genet. 1988 Jan;211(1):129-37. doi: 10.1007/BF00338403.
The entire glycerol utilization (gylABX) operon of Streptomyces coelicolor A3(2) was cloned and its transcriptional organization and regulation was analyzed by Northern blotting, S1 nuclease mapping and transcriptional fusions. Transcription of the operon is glycerol-inducible and glucose-repressible; glyA (presumptively encoding glycerol kinase), gylB (encoding sn-glycerol-3-phosphate dehydrogenase) and gylX (a non-essential 1.1 kb sequence) are transcribed consecutively to give a 5.4 kb mRNA. Two alternative transcription termination or gyl mRNA processing sites are located within the operon; one (a discrete site) lies between gylB and gylX and the other (a heterogeneous site) positioned 3 kb into the operon, may correspond to the gylA-gylB intercistronic region. A 0.9 kb glycerol-inducible transcription unit is located immediately upstream of gylABX. Transcriptional fusion studies employing an attP site-deleted phage vector provided complementary evidence for the organization of the operon.
天蓝色链霉菌A3(2)的整个甘油利用(gylABX)操纵子被克隆，并通过Northern印迹、S1核酸酶作图和转录融合分析了其转录组织和调控。该操纵子的转录受甘油诱导且受葡萄糖抑制；glyA(推测编码甘油激酶)、gylB(编码sn-甘油-3-磷酸脱氢酶)和gylX(一个非必需的1.1 kb序列)连续转录产生一个5.4 kb的mRNA。操纵子内有两个转录终止或gyl mRNA加工位点；一个(离散位点)位于gylB和gylX之间，另一个(异质位点)位于操纵子内3 kb处，可能对应于glyA-gylB基因间区域。一个0.9 kb的甘油诱导转录单元位于gylABX的紧邻上游。使用attP位点缺失的噬菌体载体进行的转录融合研究为操纵子的组织提供了补充证据。
