Ingram C, Delic I, Westpheling J
Molecular Biology Graduate Program, University of Pennsylvania, Philadelphia, USA.
J Bacteriol. 1995 Jun;177(12):3579-86. doi: 10.1128/jb.177.12.3579-3586.1995.
The regulation of carbon utilization is of central importance in the gene expression pathways for both morphological development and antibiotic production in Streptomyces species. We report the identification and characterization of a mutation in Streptomyces coelicolor, ccrA1, that affects the expression of several catabolite-controlled promoters. ccrA1 mutants are altered in expression of galP1, the glucose-sensitive, galactose-dependent promoter of the galactose utilization operon; in expression of the glycerol utilization operon, which is glucose sensitive and glycerol dependent; and in expression of chi63, the glucose-sensitive chitin-dependent promoter of a gene involved in chitin utilization. ccrA1 has no effect on the expression of galP2, a promoter that directs constitutive transcription of the galE and galK genes. ccrA1 maps to a region of the S. coelicolor genome which distinguishes it from other mutations known to be involved in catabolite control. We suggest that ccrA1 identifies a gene whose product may be involved in the general regulation of carbon catabolite repression in this complex bacterium.
碳利用的调控在链霉菌属物种的形态发育和抗生素生产的基因表达途径中至关重要。我们报告了天蓝色链霉菌中一个影响多个分解代谢物控制启动子表达的突变ccrA1的鉴定和特征。ccrA1突变体在半乳糖利用操纵子的葡萄糖敏感、半乳糖依赖性启动子galP1的表达中发生改变；在葡萄糖敏感且甘油依赖性的甘油利用操纵子的表达中发生改变；在几丁质利用相关基因的葡萄糖敏感、几丁质依赖性启动子chi63的表达中发生改变。ccrA1对指导galE和galK基因组成型转录的启动子galP2的表达没有影响。ccrA1定位于天蓝色链霉菌基因组的一个区域，这使其与已知参与分解代谢物控制的其他突变区分开来。我们认为ccrA1鉴定出了一个基因，其产物可能参与这种复杂细菌中碳分解代谢物阻遏的一般调控。
