Mende Katherina, Stuetzer Bianca, Truyen Uwe, Hartmann Katrin
Clinic of Small Animal Medicine, Ludwig-Maximilians-University of Munich, Munich, Germany
Clinic of Small Animal Medicine, Ludwig-Maximilians-University of Munich, Munich, Germany.
J Feline Med Surg. 2014 Oct;16(10):805-11. doi: 10.1177/1098612X14520812. Epub 2014 Feb 4.
Measuring antibody titres to determine a cat's immunity to core diseases instead of just administering annual vaccinations has not been established in Germany so far. An in-house test kit for the detection of antibodies against feline panleukopenia virus (FPV), feline herpesvirus-1 and feline calicivirus-- the ImmunoComb Feline VacciCheck--is now available in several European countries. The aim of this study was to assess the quality of the ImmunoComb Feline VacciCheck to determine antibodies by comparing it to a gold standard. The test is aimed for use in practice to assist decision-making when performing an individual health assessment to see whether a cat is potentially unprotected against FPV and requires FPV vaccination. Sera from 347 cats were included in the study. For antibody detection, haemagglutination inhibition (HI) was performed as gold standard. Sensitivity, specificity and positive and negative predictive values of the ImmunoComb Feline VacciCheck were determined for three different HI titre cut-off points (1:20, 1:40, 1:80). In comparison to the HI, the ImmunoComb Feline VacciCheck showed a sensitivity of 79%, 83% and 87%, and a specificity of 89%, 86% and 81%, respectively. Specificity of the ImmunoComb Feline VacciCheck, which was considered the most important parameter, was acceptable in comparison to HI. Especially when considering an antibody titre of 1:20 sufficient for protection (eg, in an adult animal), the ImmunoComb Feline VacciCheck can be recommended for use in veterinary practice.
在德国，目前尚未确立通过检测抗体滴度来确定猫对核心疾病的免疫力，而不仅仅是每年进行疫苗接种。一种用于检测抗猫泛白细胞减少症病毒（FPV）、猫疱疹病毒1型和猫杯状病毒的内部检测试剂盒——免疫组合猫疫苗检测试剂盒（ImmunoComb Feline VacciCheck），现已在几个欧洲国家上市。本研究的目的是通过将免疫组合猫疫苗检测试剂盒与金标准进行比较，评估其检测抗体的质量。该检测旨在用于实践，在进行个体健康评估以确定猫是否可能对FPV无保护且需要接种FPV疫苗时辅助决策。347只猫的血清被纳入研究。对于抗体检测，以血凝抑制试验（HI）作为金标准。针对三个不同的HI滴度截断点（1:20、1:40、1:80），确定了免疫组合猫疫苗检测试剂盒的敏感性、特异性以及阳性和阴性预测值。与HI相比，免疫组合猫疫苗检测试剂盒的敏感性分别为79%、83%和87%，特异性分别为89%、86%和81%。免疫组合猫疫苗检测试剂盒的特异性被认为是最重要的参数，与HI相比是可以接受的。特别是当认为抗体滴度为1:20足以提供保护时（例如，在成年动物中），免疫组合猫疫苗检测试剂盒可推荐用于兽医实践。
