钌红可降低青蛙神经肌肉接头处的乙酰胆碱敏感性，并增加脱敏作用。
Ruthenium red reduces acetylcholine sensitivity and increases desensitization at the frog neuromuscular junction.
作者信息
Ribera A B, Nastuk W L
机构信息
Department of Physiology, College of Physicians and Surgeons, Columbia University, New York, NY 10032.
出版信息
Neuroscience. 1987 Nov;23(2):739-44. doi: 10.1016/0306-4522(87)90091-1.
The actions of Ruthenium Red on the synaptic membrane were studied at the neuromuscular junction of the frog Rana pipiens. Ruthenium Red blocks mitochondrial calcium transport and thus is expected to elevate the intracellular calcium level [Alnaes and Rahamimoff (1975) J. Physiol., Lond. 248, 285-306]. The postjunctional membrane sensitivity was reduced by Ruthenium Red as determined by (a) the amplitude of the miniature endplate potentials, (b) iontophoretic application of carbachol, (c) microperfusion of carbachol. Desensitization was assayed by measuring the decline in the amplitude of the postsynaptic depolarization produced during repetitive iontophoretic application of carbachol. Following Ruthenium Red treatment desensitization was increased. This action of Ruthenium Red was enhanced by raising the extracellular calcium concentration from 1.8 to 10 mM.
在牛蛙（Rana pipiens）的神经肌肉接头处研究了钌红对突触膜的作用。钌红可阻断线粒体钙转运，因此预计会提高细胞内钙水平[阿尔内斯和拉哈米莫夫（1975年）《伦敦生理学杂志》248卷，285 - 306页]。通过以下方法确定，钌红可降低接头后膜敏感性：（a）微小终板电位的幅度；（b）离子电泳施加卡巴胆碱；（c）微灌流卡巴胆碱。通过测量在重复离子电泳施加卡巴胆碱期间产生的突触后去极化幅度的下降来检测脱敏情况。钌红处理后脱敏增加。将细胞外钙浓度从1.8 mM提高到10 mM可增强钌红的这种作用。
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