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缺磷条件下满江红中磷酸酶活性的变化

Changes in phosphatase activity in phosphorus-deficient Spirodela.

机构信息

Fruit Research Division, Department of Scientific and Industrial Research, Auckland, New Zealand.

出版信息

Planta. 1970 Dec;94(4):273-81. doi: 10.1007/BF00385759.

DOI:10.1007/BF00385759
PMID:24496971
Abstract

Onset of phosphorus deficiency in Spirodela oligorrhiza was accompanied by a 50-fold increase in phosphatase activity of cell extracts. The enzyme behaved like other plant acid phosphatase, and was both inhibited and repressed by inorganic phosphate. The phosphatase activity comprised at least three isozymes. Two, of low molecular weight, were present only in P-deficient Spirodela; one of high molecular weight was also present, though in smaller amounts, in normal Spirodela. Presence of 2-thiouracil during onset of P-deficiency partly inhibited the development of phosphatase activity. The nature and role of the increased phosphatase activity in P-deficient plants are discussed.

摘要

缺磷时紫萍细胞提取液中的磷酸酶活性增加了 50 倍。这种酶的性质与其他植物酸性磷酸酶相似,它既受无机磷的抑制,又受无机磷的阻遏。这种磷酸酶至少包含三种同工酶。其中两种分子量较低,仅存在于缺磷的紫萍中;另一种分子量较高,在正常紫萍中虽含量较少,但也存在。在缺磷开始时加入 2-巯基尿嘧啶,部分地抑制了磷酸酶活性的发展。本文讨论了在缺磷植物中这种增加的磷酸酶活性的性质和作用。

相似文献

1
Changes in phosphatase activity in phosphorus-deficient Spirodela.缺磷条件下满江红中磷酸酶活性的变化
Planta. 1970 Dec;94(4):273-81. doi: 10.1007/BF00385759.
2
Response of Spirodela oligorrhiza to Phosphorus Deficiency.紫萍对磷缺乏的响应。
Plant Physiol. 1970 Oct;46(4):609-13. doi: 10.1104/pp.46.4.609.
3
Turnover of Phospholipids in Normal and Phosphorus-deficient Spirodela.正常和缺磷水绵中磷脂的周转率。
Plant Physiol. 1972 May;49(5):740-5. doi: 10.1104/pp.49.5.740.
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The glycosylphosphatidylinositol-anchored phosphatase from Spirodela oligorrhiza is a purple acid phosphatase.少根紫萍的糖基磷脂酰肌醇锚定磷酸酶是一种紫色酸性磷酸酶。
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Effect of phosphorus deficiency on levels of phosphorus compounds in spirodela.缺磷对紫萍磷化合物水平的影响。
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Evidence for a glycosylinositolphospholipid-anchored alkaline phosphatase in the aquatic plant Spirodela oligorrhiza.水生植物少根紫萍中糖基磷脂酰肌醇锚定碱性磷酸酶的证据。
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Isoenzymes of acid phosphatase in germinating peas.发芽豌豆中的酸性磷酸酶同工酶。
Planta. 1973 Mar;115(1):1-10. doi: 10.1007/BF00388599.
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A procedure for the in vivo determination of enzyme activity in higher plant tissue.一种用于活体测定高等植物组织中酶活性的方法。

本文引用的文献

1
Response of Spirodela oligorrhiza to Phosphorus Deficiency.紫萍对磷缺乏的响应。
Plant Physiol. 1970 Oct;46(4):609-13. doi: 10.1104/pp.46.4.609.
2
Effect of phosphorus deficiency on levels of phosphorus compounds in spirodela.缺磷对紫萍磷化合物水平的影响。
Plant Physiol. 1968 Aug;43(8):1309-16. doi: 10.1104/pp.43.8.1309.
3
Levels of phosphate esters in spirodela.浮萍中磷酸酯的含量
Planta. 1974 Jun;118(2):133-44. doi: 10.1007/BF00388389.
4
Apyrase functions in plant phosphate nutrition and mobilizes phosphate from extracellular ATP.腺苷三磷酸双磷酸酶在植物磷营养中发挥作用,并从细胞外三磷酸腺苷中动员磷。
Plant Physiol. 1999 Feb;119(2):543-52. doi: 10.1104/pp.119.2.543.
Plant Physiol. 1968 Aug;43(8):1297-308. doi: 10.1104/pp.43.8.1297.
4
Repression of acid phosphatase synthesis in Euglena gracilis.纤细裸藻中酸性磷酸酶合成的抑制
Science. 1962 Jan 5;135(3497):46. doi: 10.1126/science.135.3497.46.
5
Preparation and some properties of an acid phosphatase from white lupine seedlings.白羽扇豆幼苗酸性磷酸酶的制备及其某些性质
Arch Biochem Biophys. 1960 Jul;89:110-7. doi: 10.1016/0003-9861(60)90020-5.
6
CYTOCHEMICAL LOCALIZATION OF ACID PHOSPHATASES IN EUGLENA GRACILIS.纤细裸藻中酸性磷酸酶的细胞化学定位
J Cell Biol. 1965 Feb;24(2):235-51. doi: 10.1083/jcb.24.2.235.
7
OBSERVATIONS ON THE ACID PHOSPHATASES OF EUGLENA GRACILIS.纤细裸藻酸性磷酸酶的观察
J Cell Biol. 1965 Feb;24(2):223-34. doi: 10.1083/jcb.24.2.223.
8
Studies on a non-specific acid phosphatase from dwarf-bean seedlings.关于矮生豆幼苗中一种非特异性酸性磷酸酶的研究。
Biochim Biophys Acta. 1962 Oct 8;64:187-9. doi: 10.1016/0006-3002(62)90776-x.
9
The effect of molybdate on the activity of tomato acid phosphatases.钼酸盐对番茄酸性磷酸酶活性的影响。
Aust J Biol Sci. 1954 May;7(2):151-60. doi: 10.1071/bi9540151.
10
A repressible alkaline phosphatase in Neurospora crassa.粗糙脉孢菌中的一种可阻遏碱性磷酸酶。
J Biol Chem. 1966 Apr 10;241(7):1468-72.