Baltaziak Marek, Wincewicz Andrzej, Kanczuga-Koda Luiza, Lotowska Joanna M, Koda Mariusz, Sulkowska Urszula, Baltaziak Marcin, Podbielski Monika, Sobaniec-Lotowska Maria E, Sulkowski Stanislaw
Department of General, Medical University of Bialystok, Poland.
Folia Histochem Cytobiol. 2013;51(4):320-5. doi: 10.5603/FHC.2013.0043.
Hypoxia triggers production of several cytoprotective proteins. Hypoxia-inducible factor 1alpha (HIF-1α) is a powerful stimulator of transcription of many genes, including erythropoietin (EPO) in hypoxia-affected cells. Recent data have also implicated signaling by EPO receptor (EPOR) as a new factor influencing tumor progression. The aim of the study was to detect by immunohistochemistry the presence of HIF-1α, EPO and EPOR in colorectal cancer (CRC) in reference to clinicopathological variables. We found the presence of the studied proteins in specimens of all 125 CRC patients which is suggestive of the occurrence of hypoxia in colorectal cancer tissues. The expression of HIF-1α correlated significantly with the presence of EPO and EPOR in all samples (P < 0.001, r = 0.549 and P < 0.001, r = 0.536, respectively). Significant correlations (from P < 0.024 to P < 0.001) were found in the analyses of CRC subgroups such as histopathological type tumor, tumor grade, tumor stage and patients with lymph nodes metastases. The same high significant correlations (P < 0.001) were observed in group of sex, age and tumor location. However, the values of the correlation coefficients (r) which usually ranged from 0.5 to 0.6 suggest the existence of independent or concurrent mechanism stimulating generation of these proteins in colorectal cancer.
缺氧会触发多种细胞保护蛋白的产生。缺氧诱导因子1α（HIF-1α）是许多基因转录的强大刺激因子，包括缺氧影响细胞中的促红细胞生成素（EPO）。最近的数据还表明，促红细胞生成素受体（EPOR）信号传导是影响肿瘤进展的一个新因素。本研究的目的是通过免疫组织化学检测结直肠癌（CRC）中HIF-1α、EPO和EPOR的存在，并参考临床病理变量进行分析。我们在所有125例CRC患者的标本中均检测到了所研究的蛋白，这表明结直肠癌组织中存在缺氧情况。在所有样本中，HIF-1α的表达与EPO和EPOR的存在均显著相关（P < 0.001，r = 0.549和P < 0.001，r = 0.536）。在对CRC亚组（如组织病理学类型肿瘤、肿瘤分级、肿瘤分期和有淋巴结转移的患者）的分析中发现了显著相关性（P < 0.024至P < 0.001）。在性别、年龄和肿瘤位置组中也观察到了同样高度显著的相关性（P < 0.001）。然而，相关系数（r）的值通常在0.5至0.6之间，这表明在结直肠癌中存在刺激这些蛋白产生的独立或并发机制。
