Departments of Soils and Plant Nutrition, of Vegetable Crops, and of Geology, University of California, Davis.
Planta. 1970 Dec;95(4):341-50. doi: 10.1007/BF00384706.
A new technique is described to prepare plant material for electron probe analysis. Root segments 1 mm in length were frozen at-170°, freeze-substituted with anhydrous ether at-30° and infiltrated with Spurr's low-viscosity epoxy resin embedding medium at low temperatures. Sections 1 and 2μ thick were cut anhydrously using hexylene glycol in the ultramicrotome trough, mounted on the polished surface of a Be disc and vacuum coated with 150-200 Å aluminum.The new technique allows retention of water-soluble ions at the original sites in the tissue and is superior to cryostat sectioning in spatial resolution of electron probe analysis and in the preservation of cellular structures.The lateral transport of K(+) into the xylem of corn roots has been successfully studied by electron probe analysis of freeze-substituted, epoxy resin embedded material.
一种新的技术被描述为准备电子探针分析的植物材料。1 毫米长的根段在-170°C 下冷冻，在-30°C 下用无水乙醚进行冷冻替代，并用低粘度的 Spurr 环氧树脂包埋介质在低温下渗透。使用在低温下的己二醇在超薄切片机槽中切割 1 和 2μ 厚的切片，安装在 Be 盘的抛光表面上，并真空镀覆 150-200Å 的铝。该新技术允许在组织中原位保留水溶性离子，在电子探针分析的空间分辨率和细胞结构的保存方面优于冷冻切片。已经成功地通过对冷冻替代的、环氧树脂包埋的材料的电子探针分析来研究 K+（钾离子）在玉米根木质部中的侧向运输。
