Kumar Yashwant, Bhatia Alka
Department of Immunopathology, PGIMER, Chandigarh, India.
Methods Mol Biol. 2014;1134:37-45. doi: 10.1007/978-1-4939-0326-9_3.
The antinuclear antibodies (ANA) also known as antinuclear factors (ANF) are unwanted molecules which bind and destroy certain structures within the nucleus. In systemic lupus erythematosus (SLE), they are produced in excess; hence their detection in the blood of patients is important for diagnosis and monitoring of the disease. Several methods are available which can be used to detect ANA; nevertheless, indirect immunofluorescence antinuclear antibody test (IF-ANA) is considered a "reference method" for their detection. Though IF-ANA is relatively easier to perform, its interpretation requires considerable skill and experience. The chapter therefore is aimed to provide comprehensive details to readers, not only about its methodology but also the result interpretation and reporting aspects of IF-ANA.
抗核抗体（ANA）也被称为抗核因子（ANF），是一些会结合并破坏细胞核内特定结构的有害分子。在系统性红斑狼疮（SLE）中，它们会过量产生；因此，在患者血液中检测到它们对于疾病的诊断和监测很重要。有几种方法可用于检测ANA；然而，间接免疫荧光抗核抗体检测（IF-ANA）被认为是检测ANA的“参考方法”。虽然IF-ANA相对容易操作，但其结果解读需要相当的技巧和经验。因此，本章旨在向读者提供全面的详细信息，不仅关于其方法，还包括IF-ANA的结果解读和报告方面。
