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Pancreatic islet isolation in rats with ductal collagenase distention, stationary digestion, and dextran separation.

作者信息

van der Vliet J A, Meloche R M, Field M J, Chen D J, Kaufman D B, Sutherland D E

机构信息

Department of Surgery, University of Minnesota, Minneapolis.

出版信息

Transplantation. 1988 Feb;45(2):493-5. doi: 10.1097/00007890-198802000-00051.

DOI:10.1097/00007890-198802000-00051
PMID:2449752
Abstract
摘要

相似文献

1
Pancreatic islet isolation in rats with ductal collagenase distention, stationary digestion, and dextran separation.
Transplantation. 1988 Feb;45(2):493-5. doi: 10.1097/00007890-198802000-00051.
2
Isolation of rat pancreatic islets by ductal injection of collagenase.
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Improved islet isolation from rat pancreas using 35% bovine serum albumin in combination with Dextran gradient separation.
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Stationary digestion after intraductal collagenase improves islet recovery in the rat.导管内注射胶原酶后进行固定消化可提高大鼠胰岛回收率。
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Improvement in islet yield from a cold-preserved pancreas by pancreatic ductal collagenase distention at the time of harvesting.在收获时通过胰管胶原酶扩张提高冷保存胰腺的胰岛产量。
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Bovine serum albumin density gradient isolation of rat pancreatic islets.牛血清白蛋白密度梯度法分离大鼠胰岛。
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A simple methodological principle for large scale extraction and purification of collagenase-digested islets.一种用于大规模提取和纯化胶原酶消化胰岛的简单方法学原理。
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8
Intraductal injection of collagenase solution at the time of harvesting: a possible solution for preservation and collagenase digestion.采集时经导管注射胶原酶溶液：一种用于保存和胶原酶消化的可能解决方案。
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Crucial role of pancreatic ductal collagenase injection for isolation of pancreatic islets.胰管胶原酶注射在胰岛分离中的关键作用。
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Use of trypsin for isolation of islets of Langerhans in the rat.
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Pancreatic ductal perfusion at organ procurement enhances islet yield in human islet isolation.
器官获取时的胰管灌注可提高人胰岛分离中的胰岛产量。
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Filtration is a time-efficient option to Histopaque, providing good-quality islets in mouse islet isolation.过滤是一种比Histopaque 更高效的选择，可为小鼠胰岛分离提供高质量的胰岛。
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