硒化修饰对大蒜多糖免疫增强活性的影响
Effects of selenylation modification on immune-enhancing activity of garlic polysaccharide.
作者信息
Qiu Shulei, Chen Jin, Qin Tao, Hu Yuanliang, Wang Deyun, Fan Qiang, Zhang Cunshuai, Chen Xingying, Chen Xiaolan, Liu Cui, Gao Zhenzhen, Li Xiuping
机构信息
Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
National Research Center of Veterinary Biological Engineering and Technology, Jiangsu Academy of Agricultural Sciences, Nanjing, China.
出版信息
PLoS One. 2014 Jan 30;9(1):e86377. doi: 10.1371/journal.pone.0086377. eCollection 2014.
The garlic polysaccharide was modified by HNO3-Na2SeO3 method according to orthogonal design L9(3(4)) to obtain nine selenizing garlic polysaccharides, sGPS1-sGPS9. Their effects on chicken peripheral lymphocytes proliferation in vitro were compared by MTT assay. The results showed that sGPSs could significantly promote lymphocytes proliferation, sGPS3, sGPS5 and sGPS6 presented stronger efficacy. In vivo experiment, 14-day-old chickens were injected respectively with sGPS3, sGPS5 and sGPS6 when they were vaccinated with ND vaccine taking unmodified GPS as control. The results showed that three sGPSs could significantly promote lymphocyte proliferation, enhance serum antibody titer, IFN-γ and IL-2 contents. These results indicated that selenylation modification could significantly enhance the immune-enhancing activity of GPS, sGPS6 possessed the best efficacy and could be as a candidate drug of immunoenhancer. Its optimal modification conditions were 400 mg of sodium selenite for 500 mg of GPS, reaction temperature of 70°C and reaction time of 6 h.
采用硝酸-亚硒酸钠法，按照正交设计L9(3(4))对大蒜多糖进行修饰，得到9种硒化大蒜多糖，即sGPS1-sGPS9。通过MTT法比较它们对鸡外周血淋巴细胞体外增殖的影响。结果表明，硒化大蒜多糖能显著促进淋巴细胞增殖，其中sGPS3、sGPS5和sGPS6的效果更强。在体内实验中，以未修饰的大蒜多糖为对照，14日龄雏鸡在接种新城疫疫苗时分别注射sGPS3、sGPS5和sGPS6。结果表明，这3种硒化大蒜多糖能显著促进淋巴细胞增殖，提高血清抗体效价、IFN-γ和IL-2含量。这些结果表明，硒化修饰能显著增强大蒜多糖的免疫增强活性，其中sGPS6效果最佳，可作为免疫增强剂的候选药物。其最佳修饰条件为：500 mg大蒜多糖加入400 mg亚硒酸钠，反应温度70℃，反应时间6 h。
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