School of Veterinary Medicine, Yangzhou University, Yangzhou 225009, PR China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, PR China; Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu 225009, PR China.
School of Veterinary Medicine, Yangzhou University, Yangzhou 225009, PR China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, PR China.
Int J Biol Macromol. 2021 Jul 1;182:136-143. doi: 10.1016/j.ijbiomac.2021.03.197. Epub 2021 Apr 5.
The purpose of this study was to optimize modification conditions of selenized garlic polysaccharides (sGPS) and investigate its structural characterization, immune and antioxidant activities. Herein, selenized garlic polysaccharides (sGPS) were prepared using by HNO-NaSeO selenylation method. And then modification conditions of sGPS were optimized through L (3) orthogonal test. The structural characterization of sGPS were identified by the Fourier-transform infrared (FT-IR), Solid-State nuclear magnetic resonance (NMR) spectra, X-ray diffraction (XRD) and thermogravimetric (TGA). The morphology of sGPS was detected using scanning electron microscope (SEM) and transmission electron microscope (TEM). In vivo investigation showed that sGPS significantly improved serum hemagglutination-inhibition (HI) antibody titers against Newcastle disease virus, enhanced secretory IgA (sIgA), IFN-γ, IL-2 secretion in jejunum and trachea irrigation compared with vaccine immunized control group. Furthermore, it showed that sGPS had some effects on the antioxidant activities in livers of chickens. In conclusion, the optimal modification conditions of sGPS were as follows: reaction temperature was 70 °C, the dosage of NaSeO was 400 mg and reaction time was 6 h. The selenylation modification of garlic polysaccharides (GPS) could improve its immune and antioxidant activity in chickens.
本研究旨在优化硒化大蒜多糖（sGPS）的修饰条件，并研究其结构特征、免疫和抗氧化活性。在此，采用 HNO-NaSeO3 硒化法制备硒化大蒜多糖（sGPS）。然后通过 L（3）正交试验优化 sGPS 的修饰条件。通过傅里叶变换红外（FT-IR）、固态核磁共振（NMR）谱、X 射线衍射（XRD）和热重分析（TGA）对 sGPS 的结构特征进行鉴定。采用扫描电子显微镜（SEM）和透射电子显微镜（TEM）检测 sGPS 的形态。体内研究表明，与疫苗免疫对照组相比，sGPS 显著提高了血清对新城疫病毒的血凝抑制（HI）抗体滴度，增强了空肠和气管灌洗液中分泌型 IgA（sIgA）、IFN-γ和 IL-2 的分泌。此外，它还表明 sGPS 对鸡肝脏的抗氧化活性有一定的影响。总之，sGPS 的最佳修饰条件如下：反应温度为 70°C，NaSeO3 的用量为 400mg，反应时间为 6h。大蒜多糖（GPS）的硒化修饰可以提高其在鸡中的免疫和抗氧化活性。
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