Feng Haibo, McDonough Sean P, Fan Jing, Yang Shiping, Zhao Xuelian, Lu Yong, Gan Yun, Yi Xiao, Chang Yung-Fu
Department of Veterinary Medicine, Southwest University, Rongchang, Chongqing 402460, China.
College of Veterinary Medicine, Cornell University, Ithaca, NY 14850, USA.
Molecules. 2017 Jan 10;22(1):106. doi: 10.3390/molecules22010106.
The aim of this study was to investigate whether phosphorylated polysaccharides (pRCPS) used as adjuvant with foot-and-mouth disease vaccine (FMDV) can stimulate specific humoral and cellular immune responses in ICR mice. The results demonstrated that pRCPS significantly up-regulated FMDV-specific IgG, IgG1, IgG2b and IgG2a antibody levels and splenocyte proliferation. pRCPS also promoted the killing activities of cytotoxic T lymphocytes (CTL) and natural killer cells (NK). In addition, pRCPS enhanced the expression levels of IL-2, IL-4, and IFN-γ in CD4⁺ T cells and the level of IFN-γ in CD8⁺ T cells. Importantly, pRCPS enhanced the expression of MHCII, CD40⁺, CD86⁺, and CD80⁺ in dendritic cells (DCs). This study indicated that phosphorylation modification could increase immune-enhancing activities of RCPS, and pRCPS could promote humoral and cellular immune responses through facilitating DC maturation.
本研究的目的是调查用作口蹄疫疫苗（FMDV）佐剂的磷酸化多糖（pRCPS）是否能刺激ICR小鼠产生特异性体液免疫和细胞免疫反应。结果表明，pRCPS显著上调了FMDV特异性IgG、IgG1、IgG2b和IgG2a抗体水平以及脾细胞增殖。pRCPS还促进了细胞毒性T淋巴细胞（CTL）和自然杀伤细胞（NK）的杀伤活性。此外，pRCPS增强了CD4⁺T细胞中IL-2、IL-4和IFN-γ的表达水平以及CD8⁺T细胞中IFN-γ的水平。重要的是，pRCPS增强了树突状细胞（DCs）中MHCII、CD40⁺、CD86⁺和CD80⁺的表达。本研究表明，磷酸化修饰可增加RCPS的免疫增强活性，且pRCPS可通过促进DC成熟来促进体液免疫和细胞免疫反应。
