磷酸化川牛膝多糖通过促进树突状细胞成熟发挥佐剂作用。
Phosphorylated Radix Cyathulae officinalis Polysaccharides Act as Adjuvant via Promoting Dendritic Cell Maturation.
作者信息
Feng Haibo, McDonough Sean P, Fan Jing, Yang Shiping, Zhao Xuelian, Lu Yong, Gan Yun, Yi Xiao, Chang Yung-Fu
机构信息
Department of Veterinary Medicine, Southwest University, Rongchang, Chongqing 402460, China.
College of Veterinary Medicine, Cornell University, Ithaca, NY 14850, USA.
出版信息
Molecules. 2017 Jan 10;22(1):106. doi: 10.3390/molecules22010106.
The aim of this study was to investigate whether phosphorylated polysaccharides (pRCPS) used as adjuvant with foot-and-mouth disease vaccine (FMDV) can stimulate specific humoral and cellular immune responses in ICR mice. The results demonstrated that pRCPS significantly up-regulated FMDV-specific IgG, IgG1, IgG2b and IgG2a antibody levels and splenocyte proliferation. pRCPS also promoted the killing activities of cytotoxic T lymphocytes (CTL) and natural killer cells (NK). In addition, pRCPS enhanced the expression levels of IL-2, IL-4, and IFN-γ in CD4⁺ T cells and the level of IFN-γ in CD8⁺ T cells. Importantly, pRCPS enhanced the expression of MHCII, CD40⁺, CD86⁺, and CD80⁺ in dendritic cells (DCs). This study indicated that phosphorylation modification could increase immune-enhancing activities of RCPS, and pRCPS could promote humoral and cellular immune responses through facilitating DC maturation.
本研究的目的是调查用作口蹄疫疫苗（FMDV）佐剂的磷酸化多糖（pRCPS）是否能刺激ICR小鼠产生特异性体液免疫和细胞免疫反应。结果表明，pRCPS显著上调了FMDV特异性IgG、IgG1、IgG2b和IgG2a抗体水平以及脾细胞增殖。pRCPS还促进了细胞毒性T淋巴细胞（CTL）和自然杀伤细胞（NK）的杀伤活性。此外，pRCPS增强了CD4⁺T细胞中IL-2、IL-4和IFN-γ的表达水平以及CD8⁺T细胞中IFN-γ的水平。重要的是，pRCPS增强了树突状细胞（DCs）中MHCII、CD40⁺、CD86⁺和CD80⁺的表达。本研究表明，磷酸化修饰可增加RCPS的免疫增强活性，且pRCPS可通过促进DC成熟来促进体液免疫和细胞免疫反应。
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