颞叶癫痫和新皮质癫痫大鼠电流分流器的癫痫发作控制
Seizure control of current shunt on rats with temporal lobe epilepsy and neocortical epilepsy.
作者信息
Zhang Limin, Liang Shuli, Zhang Guojun, Liu Zhizhong, Lv Hong, Fang Fang, Wang Yajie, Zhang Shaohui, Kang Xixiong
机构信息
Laboratory Diagnosis Center, Beijing Tiantan Hospital, Capital Medical University, Beijing, China ; Department of Neurosurgery, Capital Epilepsy Therapy Center, First Affiliated Hospital of Chinese People's Liberation Army General Hospital, Beijing, China.
Department of Neurosurgery, Capital Epilepsy Therapy Center, First Affiliated Hospital of Chinese People's Liberation Army General Hospital, Beijing, China.
出版信息
PLoS One. 2014 Jan 30;9(1):e86477. doi: 10.1371/journal.pone.0086477. eCollection 2014.
PURPOSE
To examine the effects of current shunt on rats with temporal lobe epilepsy and neocortex epilepsy.
EXPERIMENTAL DESIGN
A kainic acid (KA)-induced model of temporal lobe seizure and a penicillin-induced model of neocortical partial seizure were used in this study. Rats of each model were randomly allocated into two groups: control and model groups. The model group was further divided into the KA or penicillin group, sham conduction group and conduction group. The current shunt was realized through the implantation of a customized conduction electrode. After surgery, electroencephalogram (EEG) was recorded for two hours for each rat under anesthesia. Subsequently, the rats were video monitored for 72 h to detect the occurrence of behavioral seizures upon awakening. The average number and duration of seizures on EEG and the number of behavioral seizures were measured.
RESULTS
In KA model, the number of total EEG seizures in conduction group (9.57±2.46) was significantly less than that in sham conduction group (15.13±3.45) (p<0.01). The duration of EEG seizures in conduction group (26.13±7.81 s) was significantly shorter than that in sham conduction group (34.17±7.25 s) (p = 0.001). A significant reduction of behavioral seizures was observed in the conduction group compared with KA (p = 0.000) and sham conduction groups (p = 0.000). In penicillin model, there was a 61% reduction in total EEG seizures in conduction group compared with sham conduction group (p<0.01), and the duration of EEG seizures in conduction group (6.29±2.64 s) was significantly shorter than that in the sham conduction group (12.07±3.81 s) (p = 0.002). A significant reduction of behavioral seizures was observed in conduction group compared with penicillin (p<0.01) and sham conduction groups (p<0.01).
CONCLUSION
Current shunt effectively reduces the onset and severity of seizures. Current shunt therapy could be an effective alternative minimally invasive approach for temporal lobe epilepsy and neocortex epilepsy.
目的
研究电流分流对颞叶癫痫和新皮质癫痫大鼠的影响。
实验设计
本研究采用 kainic 酸（KA）诱导的颞叶癫痫模型和青霉素诱导的新皮质部分性癫痫模型。每个模型的大鼠随机分为两组：对照组和模型组。模型组进一步分为 KA 或青霉素组、假传导组和传导组。通过植入定制的传导电极实现电流分流。手术后，在麻醉状态下对每只大鼠记录两小时脑电图（EEG）。随后，对大鼠进行 72 小时视频监测，以检测清醒时行为性癫痫发作的发生情况。测量 EEG 上癫痫发作的平均次数和持续时间以及行为性癫痫发作的次数。
结果
在 KA 模型中，传导组 EEG 癫痫发作的总次数（9.57±2.46）明显少于假传导组（15.13±3.45）（p<0.01）。传导组 EEG 癫痫发作的持续时间（26.13±7.81 秒）明显短于假传导组（34.17±7.25 秒）（p = 0.001）。与 KA 组（p = 0.000）和假传导组（p = 0.000）相比，传导组行为性癫痫发作明显减少。在青霉素模型中，与假传导组相比，传导组 EEG 癫痫发作的总次数减少了 61%（p<0.01），传导组 EEG 癫痫发作的持续时间（6.29±2.64 秒）明显短于假传导组（12.07±3.81 秒）（p = 0.002）。与青霉素组（p<0.01）和假传导组（p<0.01）相比，传导组行为性癫痫发作明显减少。
结论
电流分流有效降低癫痫发作的起始和严重程度。电流分流疗法可能是颞叶癫痫和新皮质癫痫一种有效的微创替代方法。
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