Raedt R, Van Dycke A, Van Melkebeke D, De Smedt T, Claeys P, Wyckhuys T, Vonck K, Wadman W, Boon P
Laboratory for Clinical and Experimental Neurophysiology, Department of Neurology, University Hospital Ghent, De Pintelaan 185, Ghent, Belgium.
Acta Neurol Scand. 2009 May;119(5):293-303. doi: 10.1111/j.1600-0404.2008.01108.x.
Intrahippocampal injection of kainic acid (KA) in rats evokes a status epilepticus (SE) and leads to spontaneous seizures. However to date, precise electroencephalographic (EEG) and clinical characterization of spontaneous seizures in this epilepsy model using long-term video-EEG monitoring has not been performed.
Rats were implanted with bipolar hippocampal depth electrodes and a cannula for the injection of KA (0.4 lg /0.2 ll) in the right hippocampus. Video-EEG monitoring was used to determine habitual parameters of spontaneous seizures such as seizure frequency, severity, progression and day-night rhythms.
Spontaneous seizures were detected in all rats with 13 out of 15 animals displaying seizures during the first eight weeks after SE. A considerable fraction (35%) of the spontaneous seizures did not generalize secondarily. Seizure frequency was quite variable and the majority of the KA treated animals had less than one seizure per day. A circadian rhythm was observed in all rats that showed sufficient seizures per day.
This study shows that the characteristics of spontaneous seizures in the intrahippocampal KA model display many similarities to other SE models and human temporal lobe epilepsy.
向大鼠海马内注射红藻氨酸（KA）可诱发癫痫持续状态（SE）并导致自发性癫痫发作。然而，迄今为止，尚未使用长期视频脑电图监测对该癫痫模型中自发性癫痫发作进行精确的脑电图（EEG）和临床特征描述。
给大鼠植入双极海马深度电极以及用于在右侧海马注射KA（0.4μg/0.2μl）的套管。使用视频脑电图监测来确定自发性癫痫发作的习惯参数，如发作频率、严重程度、进展情况和昼夜节律。
在所有大鼠中均检测到自发性癫痫发作，15只动物中有13只在SE后的前八周内出现癫痫发作。相当一部分（35%）的自发性癫痫发作并未继发全身性发作。癫痫发作频率变化很大，大多数接受KA治疗的动物每天癫痫发作少于一次。在所有每天有足够癫痫发作次数的大鼠中均观察到昼夜节律。
本研究表明，海马内KA模型中自发性癫痫发作的特征与其他SE模型和人类颞叶癫痫有许多相似之处。
