Entomology Laboratory, Faculty of Biology, Gadjah Mada University. Jalan Teknika Selatan, Sekip Utara, Depok Sleman 55281, Indonesia.
Biochemistry Laboratory, Faculty of Biology, Gadjah Mada University, Jalan Teknika Selatan, Sekip Utara Depok Sleman 55281, Indonesia.
J Econ Entomol. 2013 Dec;106(6):2364-70. doi: 10.1603/ec13144.
The objective of this research was to evaluate artificial diets that can be used to successfully culture the atlas silk moth, Attacus atlas L. (Lepidoptera: Saturniidae) indoors. Four plant species were evaluated as the basic component of each diet, barringtonia (Barringtonia asiatica), cheesewood (Nauclea orientalis), soursop (Annona muricata), and mahogany (Swietenia mahagoni). Evaluation of the nutritional value of each diet was determined by an analysis of the hemolymph proteins of sixth instars using the Folin-Ciocalteu assay. Survivorship, cocoon quality, and hemolymph protein content of larvae fed the barringtonia diet were higher than those of larvae fed mahogany-, cheesewood-, and soursop-based artificial diets. The average adult emergence of those fed the barringtonia-based diet was 74.5%. The weights of the cocoon in this treatment with the pupa and the empty cocoons were 7.0 and 1.1 g, respectively. Hemolymph of the larvae fed the barringtonia-based artificial diet had the highest concentration of protein with an average of 28.06 mg/ml. The atlas moth reared on the barringtonia-based artificial diet was comparable with those reared only on barringtonia leaves. However, the weight of empty cocoons, adult wingspan, and amount of hemolymph protein were lower than in those reared on barringtonia leaves only. This may suggest that the artificial barringtonia-based diet requires additional protein for maximum efficiency.
本研究旨在评估可成功用于室内饲养大蚕蛾（Attacus atlas L.）的人工饲料。评估了四种植物作为每种饲料的基本成分，包括水黄皮（Barringtonia asiatica）、诃子（Nauclea orientalis）、杨桃（Annona muricata）和桃花心木（Swietenia mahagoni）。通过福林-酚试剂法分析六龄幼虫的血淋巴蛋白来评估每种饲料的营养价值。用水黄皮饲料饲养的幼虫的存活率、茧质和血淋巴蛋白含量均高于用桃花心木、诃子和杨桃基人工饲料饲养的幼虫。用水黄皮基人工饲料饲养的幼虫的平均成虫羽化率为 74.5%。该处理的蛹和空茧的平均茧重分别为 7.0 和 1.1 克。用水黄皮基人工饲料饲养的幼虫的血淋巴蛋白浓度最高，平均为 28.06 毫克/毫升。用水黄皮基人工饲料饲养的大蚕蛾与仅用水黄皮叶饲养的大蚕蛾相当。然而，空茧重量、成虫翅展和血淋巴蛋白含量均低于仅用水黄皮叶饲养的大蚕蛾。这可能表明，这种人工水黄皮基饲料需要额外的蛋白质才能达到最高效率。
