El-Said Saad Mohamed
Buraydah College of Food and Environment Technology, Buraydah, Saudi Arabia.
Pak J Biol Sci. 2013 Mar 15;16(6):287-90. doi: 10.3923/pjbs.2013.287.290.
There are pesticide residues in agriculture wastewater and that compounds must be removed before discharge of wastewater in native waters. Thus the aim of this study was to remove toxic pesticide in waste water by the addition of horseradish peroxidase enzyme. The process of pesticide (methyl-parathion (O,O-Diethyl- O-4-nitro-phenylthiophosphate), atrazine (1-chloro-3-ethylamino-5-isopropylamino-2,4,6-triazine) and triazophos (O,O-diethyl O-1-phenyl-1H-1,2,4- triazol-3-yl phosphorothioate) removal from synthetic wastewater using horseradish peroxidase and hydrogen peroxide has been analyzed. The technical feasibility of the process was studied using 0.001-3.0 mM synthetic pesticides solutions. Experiments were carried out at different time, HRP and H2O2 dose and pH to determine the optimum removing conditions. The removal of the three pesticides increases with an increase in HRP and hydrogen peroxide dose. The optimum HRP dose is 2.0 U L(-1) and 10 mM for H2O2. The contact needed to reach equilibrium was found to be 360 min. Maximum removal was achieved up to 74% at pH 8. Also, Chemical Oxygen Demand (COD) of the effluent reduced at the end of 6 h from 2111-221 mg L(-1) (at pH 8). Tests based upon horseradish peroxidase, at optimized parameters, show the reduction of toxicity to non-toxic levels.
农业废水中存在农药残留，在将废水排放到天然水体之前必须去除这些化合物。因此，本研究的目的是通过添加辣根过氧化物酶来去除废水中的有毒农药。分析了使用辣根过氧化物酶和过氧化氢从合成废水中去除农药（甲基对硫磷（O,O-二乙基-O-4-硝基苯硫代磷酸酯）、阿特拉津（1-氯-3-乙基氨基-5-异丙基氨基-2,4,6-三嗪）和三唑磷（O,O-二乙基-O-1-苯基-1H-1,2,4-三唑-3-基硫代磷酸酯））的过程。使用0.001 - 3.0 mM的合成农药溶液研究了该过程的技术可行性。在不同时间、辣根过氧化物酶和过氧化氢剂量以及pH值下进行实验，以确定最佳去除条件。三种农药的去除率随着辣根过氧化物酶和过氧化氢剂量的增加而增加。最佳辣根过氧化物酶剂量为2.0 U L(-1)，过氧化氢为10 mM。发现达到平衡所需的接触时间为360分钟。在pH 8时，最大去除率可达74%。此外，在6小时结束时，出水的化学需氧量（COD）从2111 - 221 mg L(-1)（在pH 8时）降低。基于辣根过氧化物酶的测试，在优化参数下，显示毒性降低到无毒水平。
