Auriol Muriel, Filali-Meknassi Youssef, Adams Craig D, Tyagi Rajeshwar D, Noguerol Tania-Noelia, Piña Benjamin
University of Missouri-Rolla, Environmental Research Center of Emerging Contaminants, 220 Butler Carlton Hall, Rolla, MO 65409, USA.
Chemosphere. 2008 Jan;70(3):445-52. doi: 10.1016/j.chemosphere.2007.06.064. Epub 2007 Sep 25.
Some researches studied the removal of steroid estrogens by enzymatic treatment, however none verified the residual estrogenicity after the enzymatic treatment at environmental conditions. In this study, the residual estrogenic activities of the key natural and synthetic steroid estrogens were investigated following enzymatic treatment with horseradish peroxidase (HRP) and laccase from Trametes versicolor. Synthetic water and municipal wastewater containing environmental concentrations of estrone, 17beta-estradiol, estriol, and 17alpha-ethinylestradiol were treated. Liquid chromatography-mass spectrometry analysis demonstrated that the studied steroid estrogens were completely oxidized in the wastewater reaction mixture after a 1-h treatment with either HRP (8-10 U ml(-1)) or laccase (20 U ml(-1)). Using the recombinant yeast assay, it was also confirmed that both enzymatic treatments were very efficient in removing the estrogenic activity of the studied steroid estrogens. The laccase-catalyzed process seemed to present great advantages over the HRP-catalyzed system for up-scale applications for the treatment of municipal wastewater.
一些研究探讨了通过酶处理去除类固醇雌激素的方法，然而，尚无研究验证在环境条件下酶处理后雌激素的残留情况。在本研究中，用辣根过氧化物酶（HRP）和云芝漆酶对关键的天然和合成类固醇雌激素进行酶处理后，研究了其残留雌激素活性。对含有环境浓度的雌酮、17β-雌二醇、雌三醇和17α-乙炔基雌二醇的合成水和城市废水进行了处理。液相色谱-质谱分析表明，在用HRP（8-10 U ml(-1)）或漆酶（20 U ml(-1)）处理1小时后，研究的类固醇雌激素在废水反应混合物中完全被氧化。使用重组酵母试验还证实，两种酶处理在去除所研究类固醇雌激素的雌激素活性方面都非常有效。对于城市废水处理的大规模应用而言，漆酶催化过程似乎比HRP催化系统具有更大优势。
