Bodnar Lubomir, Stanczak Aleksandra, Cierniak Szczepan, Smoter Marta, Cichowicz Marzena, Kozlowski Wojciech, Szczylik Cezary, Wieczorek Maciej, Lamparska-Przybysz Monika
Department of Oncology, Military Institute of Medicine in Warsaw, 128 Szaserów Street, 04-141 Warsaw, Poland.
J Ovarian Res. 2014 Feb 6;7:16. doi: 10.1186/1757-2215-7-16.
β-catenin is the key protein in the WNT signalling pathway and it forms adherent junctions together with E-cadherin. In ovarian carcinoma, abnormal expression of β-catenin, E-cadherin and WNT-1 was observed, but their prognostic and predictive role is unclear. The aim of this study was to clarify the prognostic and predictive role of E-cadherin, β-catenin and WNT-1 in advanced epithelial ovarian carcinoma (AEOC).
The expression of E-cadherin, β-catenin and WNT-1 was determined by immunohistochemistry in AEOC. The correlation between expression of these proteins and progression-free survival (PFS) and overall survival (OS) was evaluated. Statistical analyses included Kaplan-Meier estimation, log-rank test, Spearman correlation and Cox proportional-hazards model.
In ovarian cancer, intense expression of E-cadherin, β-catenin and WNT-1 was found. In multivariate analysis, strong membrane β-catenin expression was an independent unfavourable predictor for PFS (HR 2.19, 95% CI 1.09-4.39; p = 0.028), while in univariate analysis, strong membrane β-catenin expression was a prognostic factor for OS in patients with AOC (p = 0.039). In multivariate analysis, only resistance to first-line chemotherapy was an adverse independent prognostic factor for OS (HR 16.84; 95% CI 5.07-55.98; p < 0.0001). Additionally, strong membranous β-catenin expression was associated with resistance to platinum-based chemotherapy (p = 0.027).
These findings support that WNT/β-catenin pathway and E-cadherin are important factors in advanced epithelial ovarian cancer.
β-连环蛋白是WNT信号通路中的关键蛋白，它与E-钙黏蛋白共同形成黏附连接。在卵巢癌中，观察到β-连环蛋白、E-钙黏蛋白和WNT-1的异常表达，但其预后和预测作用尚不清楚。本研究的目的是阐明E-钙黏蛋白、β-连环蛋白和WNT-1在晚期上皮性卵巢癌（AEOC）中的预后和预测作用。
通过免疫组织化学法检测AEOC中E-钙黏蛋白、β-连环蛋白和WNT-1的表达。评估这些蛋白表达与无进展生存期（PFS）和总生存期（OS）之间的相关性。统计分析包括Kaplan-Meier估计、对数秩检验、Spearman相关性分析和Cox比例风险模型。
在卵巢癌中，发现E-钙黏蛋白、β-连环蛋白和WNT-1有强烈表达。多因素分析中，β-连环蛋白强膜表达是PFS的独立不良预测因素（HR 2.19，95%CI 1.09-4.39；p = 0.028），而单因素分析中，β-连环蛋白强膜表达是AOC患者OS的预后因素（p = 0.039）。多因素分析中，仅对一线化疗的耐药性是OS的不良独立预后因素（HR 16.84；95%CI 5.07-55.98；p < 0.0001）。此外，β-连环蛋白强膜表达与铂类化疗耐药相关（p = 0.027）。
这些发现支持WNT/β-连环蛋白通路和E-钙黏蛋白是晚期上皮性卵巢癌的重要因素。
