Kira J, Itoyama Y, Goto I
Department of Neurology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Cell Immunol. 1988 Mar;112(1):14-26. doi: 10.1016/0008-8749(88)90272-9.
The relationship between surface marker expression and encephalitogenicity of lymphocytes from various lymphoid organs of Lewis rats was studied. The encephalitogenicities after culture with BP were spleen cells greater than lymph node cells much greater than thymus cells, in this descending order. The cells from every lymphoid organ proliferated significantly in response to BP. In spleen and lymph node cells, the expression of W3/25 and OX-3 molecules on T cells increased markedly after culture with BP, but the expression of OX-19 or OX-8 molecules did not change significantly. The up-regulations of W3/25 and OX-3 molecules were more pronounced in spleen cells than in lymph node cells. Thymus cells also showed a significant increase in the W3/25 molecule after the culture with BP. Therefore, T cells from all the lymphoid organs showed a selective up-regulation of the W3/25 molecule after culture with BP, and the degree of the up-regulation seems to correspond to the encephalitogenic potency in vivo. Since the W3/25 molecule apparently plays a direct role in the effector phase of experimental allergic encephalomyelitis (EAE) by enhancing BP-reactive T cell/antigen-presenting cell interaction in the central nervous system, the up-regulation on BP-cultured T cells may strengthen interaction with the class II major histocompatibility complex molecule on antigen-presenting cells, and therefore, contribute to the efficient transfer of EAE.
研究了Lewis大鼠不同淋巴器官淋巴细胞表面标志物表达与致脑炎性之间的关系。用BP培养后的致脑炎性依次为脾细胞大于淋巴结细胞远大于胸腺细胞。每个淋巴器官的细胞对BP均有显著增殖反应。在脾细胞和淋巴结细胞中，用BP培养后T细胞上W3/25和OX-3分子的表达明显增加，但OX-19或OX-8分子的表达无显著变化。W3/25和OX-3分子的上调在脾细胞中比在淋巴结细胞中更明显。胸腺细胞在用BP培养后W3/25分子也有显著增加。因此，所有淋巴器官的T细胞在用BP培养后均表现出W3/25分子的选择性上调，上调程度似乎与体内致脑炎性效力相对应。由于W3/25分子显然通过增强中枢神经系统中BP反应性T细胞/抗原呈递细胞的相互作用在实验性变应性脑脊髓炎（EAE）的效应阶段发挥直接作用，BP培养的T细胞上的上调可能会加强与抗原呈递细胞上II类主要组织相容性复合体分子的相互作用，因此有助于EAE的有效传递。
