Kira J, Itoyama Y, Goto I
Department of Neurology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Cell Immunol. 1989 Oct 15;123(2):264-75. doi: 10.1016/0008-8749(89)90287-6.
The relationship between surface molecule expression and encephalitogenicity of myelin basic protein (BP)-sensitized cells induced by three different sensitization protocols was studied using adoptive transfer in Lewis rats. (i) In BP/CFA sensitization, CD4+ blastoid T cells showing marked upregulation of CD4, class I and II MHC, and IL2 receptor molecules, but not CD5, CD8, or CD45, were generated after culture with BP. In this case, BP-cultured cells were strongly encephalitogenic in the recipients. (ii) In the case of BP/IFA sensitization, CD4+ T cells showed no remarkable change of cell size or surface molecule expression after culture with BP and were weakly encephalitogenic in the recipients. Vigorous proliferation of the cells induced by addition of recombinant IL2 to the culture with BP neither enhanced the encephalitogenicity nor produced CD4+ blastoid T cells showing marked upregulation of CD4, class I and II MHC, and IL2 receptor molecules. (iii) The sequentially transferred naive T cells showed no remarkable change of cell size or surface molecule expression, even after a second culture with BP, and were the least encephalitogenic. These data suggest that the generation of CD4+ blastoid T cells showing marked upregulation of CD4, class I and II MHC, and IL2 receptor molecules but not vigorous proliferation correlates closely with the potent encephalitogenicity in vivo.
采用过继转移法，在Lewis大鼠中研究了三种不同致敏方案诱导的髓鞘碱性蛋白（BP）致敏细胞的表面分子表达与致脑炎能力之间的关系。（i）在BP/CFA致敏中，与BP培养后产生了CD4+母细胞样T细胞，这些细胞的CD4、I类和II类MHC以及IL2受体分子显著上调，但CD5、CD8或CD45未上调。在这种情况下，BP培养的细胞在受体中具有强烈的致脑炎能力。（ii）在BP/IFA致敏的情况下，CD4+T细胞与BP培养后细胞大小和表面分子表达没有明显变化，在受体中致脑炎能力较弱。在与BP的培养物中添加重组IL2诱导细胞大量增殖，既没有增强致脑炎能力，也没有产生CD4、I类和II类MHC以及IL2受体分子显著上调的CD4+母细胞样T细胞。（iii）即使在与BP进行第二次培养后，顺序转移的初始T细胞的细胞大小和表面分子表达也没有明显变化，并且其致脑炎能力最弱。这些数据表明，产生CD4、I类和II类MHC以及IL2受体分子显著上调但不大量增殖的CD4+母细胞样T细胞与体内强大的致脑炎能力密切相关。
