Kalina M, Riklis S
Department of Histology and Cell Biology, Sackler School of Medicine, Tel Aviv University, Israel.
Histochemistry. 1988;88(2):175-9. doi: 10.1007/BF00493301.
The development and characteristics of rat alveolar type II cells were monitored by using various cytochemical techniques. Polarized light microscopy was found useful for observing live type II cells in culture. Cells progressively lose their birefringent granules starting from 48 h of cells in culture, indicating the disappearance of the phospholipids organized lamellae in the lamellar bodies. Similar results were obtained by using an immunocytochemical approach with antibodies raised against the apoprotein component of rat surfactant. A progressive decrease in immuno-staining corresponded to the disappearance of the lamellar bodies, and birefringence. Changes in lectins binding to the cultured type II cells were also observed. Freshly isolated and one day old cultured cells could bind Macula pomifera (MPA) but not Arachis hypogaea (PA) lectins. The reverse was found in 6-7 days old cultured cells which had the ability to bind PA but not MPA the advantage of using various cytochemical techniques for studying the development of type II cells in culture is being discussed.
通过使用各种细胞化学技术监测大鼠肺泡II型细胞的发育和特征。发现偏振光显微镜对于观察培养中的活II型细胞很有用。从培养48小时起，细胞逐渐失去其双折射颗粒，这表明板层小体中磷脂组织的片层消失。通过使用针对大鼠表面活性剂载脂蛋白成分产生的抗体的免疫细胞化学方法也获得了类似的结果。免疫染色的逐渐减少与板层小体的消失和双折射相对应。还观察到凝集素与培养的II型细胞结合的变化。新鲜分离的和培养一天的细胞可以结合美洲商陆（MPA）凝集素，但不能结合花生（PA）凝集素。在培养6-7天的细胞中发现相反的情况，这些细胞能够结合PA但不能结合MPA。正在讨论使用各种细胞化学技术研究培养中的II型细胞发育的优势。
