Dobbs L G, Williams M C, Brandt A E
Biochim Biophys Acta. 1985 Jul 30;846(1):155-66. doi: 10.1016/0167-4889(85)90121-1.
When cultured on plastic culture dishes for several days, alveolar type II cells gradually lose both their morphologic and biochemical identifying characteristics. Although type II cells cultured on a matrix derived from corneal endothelial cells have previously been reported to retain lamellar bodies for 7-10 days in culture, the ability of type II cells cultured on matrix to synthesize surfactant lipids has not been previously studied. We therefore measured the phospholipid content and the distribution of [14C]acetate into classes of lipids by type II cells maintained in culture. We found no differences between cells cultured on plastic or on matrix. We then studied the binding to type II cells in culture of Maclura pomifera and Ricinus communis I, lectins specific in vivo for type II and type I cells, respectively. We found that the cells progressively bind less M. pomifera and more R. communis I. The change in pattern of lectin binding occurs whether cells are cultured on plastic or matrix, whether lectins are conjugated with fluorescein, rhodamine or ferritin, or whether cells are cultured in the presence or absence of serum. We conclude that type II cells cultured on either tissue culture plastic or matrix derived from corneal endothelial cells lose the ability to synthesize and contain surfactant phospholipids, and, at least in their pattern of lectin binding, become similar to type I cells.
当在塑料培养皿中培养数天后，肺泡II型细胞会逐渐丧失其形态学和生化识别特征。尽管此前有报道称，在源自角膜内皮细胞的基质上培养的II型细胞在培养7至10天内可保留板层小体，但此前尚未研究过在基质上培养的II型细胞合成表面活性物质脂质的能力。因此，我们测量了培养的II型细胞中磷脂含量以及[14C]乙酸盐在脂质类别中的分布情况。我们发现，在塑料或基质上培养的细胞之间没有差异。然后，我们研究了桑橙凝集素和蓖麻凝集素I与培养的II型细胞的结合情况，这两种凝集素在体内分别对II型和I型细胞具有特异性。我们发现，细胞与桑橙凝集素的结合逐渐减少，而与蓖麻凝集素I的结合逐渐增加。无论细胞是在塑料还是基质上培养，无论凝集素是与荧光素、罗丹明还是铁蛋白偶联，也无论细胞是在有血清还是无血清的情况下培养，凝集素结合模式都会发生变化。我们得出结论，在组织培养塑料或源自角膜内皮细胞的基质上培养的II型细胞丧失了合成和包含表面活性物质磷脂的能力，并且至少在其凝集素结合模式上，变得与I型细胞相似。
