Amara S, Slama I Ben, Mrad I, Rihane N, Khemissi W, El Mir L, Rhouma K Ben, Abdelmelek H, Sakly M
Laboratoire de Physiologie Intégrée, Faculté des Sciences de Bizerte, Jarzouna, Tunisia
Laboratoire de Physiologie Intégrée, Faculté des Sciences de Bizerte, Jarzouna, Tunisia.
Hum Exp Toxicol. 2014 Nov;33(11):1150-7. doi: 10.1177/0960327113510327. Epub 2014 Feb 5.
The aim of this study was to assess the potential subacute toxicity of zinc oxide (ZnO) nanoparticles (NPs) in Wistar rats in comparison with reference toxicant, zinc chloride (ZnCl2), of a non-nanoparticulate form. We therefore studied the relationships between zinc (Zn) accumulation, liver and kidney trace element levels, and plasmatic biochemical parameters. Rats in all groups were treated by intraperitoneal injection of ZnO NPs and/or ZnCl2 solution (25 mg/kg) every other day for 10 days. The contents of trace element in the liver and kidney were slightly modulated after ZnO NPs and/or ZnCl2 solution exposure. The same treatment increased the aspartate aminotransferase activity and uric acid concentration. However, ZnO NPs or ZnCl2 solution decreased the creatinine levels, whereas the combined intake of ZnO NPs and ZnCl2 decreased the glucose concentration. Interestingly, the analysis of the lyophilized powder of liver using the x-ray diffractometer showed the degradation of ZnO NPs in ZnO-treated group, instead there is a lack of NPs ZnO biosynthesis from the ZnCl2 solution injected in rats. These investigations suggest that combined injection of ZnO NPs and ZnCl2 solution has a possible toxic effect in rats. This effect could be related to Zn(2+) ion release and accumulation of this element in organs. Our findings provide crucial information that ZnO appeared to be absorbed in the organs in an ionic form rather than in a particulate form.
本研究的目的是评估氧化锌（ZnO）纳米颗粒（NPs）对Wistar大鼠的潜在亚急性毒性，并与非纳米颗粒形式的参考毒物氯化锌（ZnCl2）进行比较。因此，我们研究了锌（Zn）积累、肝脏和肾脏微量元素水平以及血浆生化参数之间的关系。所有组的大鼠每隔一天通过腹腔注射ZnO NPs和/或ZnCl2溶液（25 mg/kg）进行处理，持续10天。在暴露于ZnO NPs和/或ZnCl2溶液后，肝脏和肾脏中的微量元素含量略有变化。相同的处理增加了天冬氨酸转氨酶活性和尿酸浓度。然而，ZnO NPs或ZnCl2溶液降低了肌酐水平，而同时摄入ZnO NPs和ZnCl2则降低了葡萄糖浓度。有趣的是，使用X射线衍射仪对肝脏冻干粉末进行分析表明，在ZnO处理组中ZnO NPs发生了降解，相反，在注射了ZnCl2溶液的大鼠中没有ZnO NPs生物合成。这些研究表明，联合注射ZnO NPs和ZnCl2溶液对大鼠可能有毒性作用。这种作用可能与Zn(2+)离子释放以及该元素在器官中的积累有关。我们的研究结果提供了关键信息，即ZnO似乎是以离子形式而非颗粒形式被器官吸收。
