Karadas S, Aslan M, Gonullu H, Kati C, Duran L, Olmez S, Kucukoglu M E, Demir H
Department of Emergency Medicine, Faculty of Medicine, Yuzuncu Yil University, Van, Turkey.
Department of Internal Medicine, Faculty of Medicine, Yuzuncu Yil University, Van, Turkey
Hum Exp Toxicol. 2014 Nov;33(11):1134-40. doi: 10.1177/0960327113511477. Epub 2014 Feb 5.
Acetaminophen is at present one of the most commonly used analgesics and antipyretics. Recent evidence has suggested that oxidative stress is involved in the mechanism of acetaminophen intoxication. Paraoxonase-1 (PON1) plays an important role as an endogenous free-radical scavenging molecule. The aim of this study was to evaluate the influence of serum PON1 activity and oxidative stress in patients with acetaminophen intoxication.
A total of 20 patients with acetaminophen intoxication and 25 healthy controls were enrolled. Serum total antioxidant capacity (TAC), lipid hydroperoxide (LOOH) levels, and paraoxonase and arylesterase activities were measured spectrophotometrically.
The serum TAC levels and the paraoxonase and arylesterase activities were significantly lower in patients with acetaminophen intoxication compared with controls (all, p < 0.001), while the serum LOOH levels were significantly higher (p < 0.001).
Our results suggest that decreased PON1 activity seems to be associated with increased oxidative stress in patients with acetaminophen intoxication. Measuring serum PON1 activity may be useful in assessing the development of toxicity risk in acetaminophen toxicity. It would be useful to recommend vitamins with antioxidant effects such as vitamins C and E along with medical treatments.
对乙酰氨基酚是目前最常用的止痛和退热药物之一。最近有证据表明，氧化应激参与了对乙酰氨基酚中毒的机制。对氧磷酶-1（PON1）作为一种内源性自由基清除分子发挥着重要作用。本研究的目的是评估对乙酰氨基酚中毒患者血清PON1活性和氧化应激的影响。
共纳入20例对乙酰氨基酚中毒患者和25例健康对照者。采用分光光度法测定血清总抗氧化能力（TAC）、脂质过氧化氢（LOOH）水平以及对氧磷酶和芳基酯酶活性。
与对照组相比，对乙酰氨基酚中毒患者的血清TAC水平、对氧磷酶和芳基酯酶活性显著降低（均P<0.001），而血清LOOH水平显著升高（P<0.001）。
我们的结果表明，对乙酰氨基酚中毒患者PON1活性降低似乎与氧化应激增加有关。检测血清PON1活性可能有助于评估对乙酰氨基酚中毒时毒性风险的发展。在药物治疗的同时推荐使用具有抗氧化作用的维生素如维生素C和维生素E可能会有帮助。
