Kruse Thomas, Maillard Julien, Goodwin Lynne, Woyke Tanja, Teshima Hazuki, Bruce David, Detter Chris, Tapia Roxanne, Han Cliff, Huntemann Marcel, Wei Chia-Lin, Han James, Chen Amy, Kyrpides Nikos, Szeto Ernest, Markowitz Victor, Ivanova Natalia, Pagani Ioanna, Pati Amrita, Pitluck Sam, Nolan Matt, Holliger Christof, Smidt Hauke
Wageningen University, Agrotechnology and Food Sciences, Laboratory of Microbiology, Dreijenplein 10, NL-6703 HB Wageningen, The Netherlands.
Ecole Polytechnique Fédérale de Lausanne (EPFL), School of Architecture, Civil and Environmental Engineering, Laboratory for Environmental Biotechnology, Station 6, CH-1015 Lausanne, Switzerland.
Stand Genomic Sci. 2013 Jul 30;8(3):375-88. doi: 10.4056/sigs.3787426.
Dehalobacter restrictus strain PER-K23 (DSM 9455) is the type strain of the species Dehalobacter restrictus. D. restrictus strain PER-K23 grows by organohalide respiration, coupling the oxidation of H2 to the reductive dechlorination of tetra- or trichloroethene. Growth has not been observed with any other electron donor or acceptor, nor has fermentative growth been shown. Here we introduce the first full genome of a pure culture within the genus Dehalobacter. The 2,943,336 bp long genome contains 2,826 protein coding and 82 RNA genes, including 5 16S rRNA genes. Interestingly, the genome contains 25 predicted reductive dehalogenase genes, the majority of which appear to be full length. The reductive dehalogenase genes are mainly located in two clusters, suggesting a much larger potential for organohalide respiration than previously anticipated.
限制脱卤杆菌菌株PER-K23（DSM 9455）是限制脱卤杆菌物种的模式菌株。限制脱卤杆菌菌株PER-K23通过有机卤化物呼吸进行生长，将H2的氧化与四氯乙烯或三氯乙烯的还原脱氯作用相偶联。未观察到其利用任何其他电子供体或受体进行生长，也未显示出其进行发酵生长。在此，我们介绍了脱卤杆菌属内纯培养物的首个完整基因组。这个长度为2,943,336 bp的基因组包含2,826个蛋白质编码基因和82个RNA基因，其中包括5个16S rRNA基因。有趣的是，该基因组包含25个预测的还原脱卤酶基因，其中大多数似乎是全长的。还原脱卤酶基因主要位于两个基因簇中，这表明有机卤化物呼吸的潜力比之前预期的要大得多。
