Bruce G, Hersh L B
Department of Biochemistry, University of Texas Health Science Center, Dallas 75235.
Neurochem Res. 1987 Dec;12(12):1059-66. doi: 10.1007/BF00971705.
The relationship between soluble and membrane choline acetyltransferase (ChAT) was studied. Differential solubilization of rat and human brain yielded ChAT in the soluble and membrane fractions. The addition of 1% Triton X-100 to membrane fractions resulted in a release of ChAT. A comparable release of lactate dehydrogenase was also observed. The Triton released ChAT and soluble ChAT from rat and human brain were efficiently purified by immuno-affinity chromatography. A single molecular weight of 68,000 was observed for both forms of rat and human brain ChAT. Epitope maps produced from both forms of human brain ChAT were identical. It is concluded that Triton released ChAT is identical to soluble ChAT and simply represents occluded soluble ChAT.
研究了可溶性和膜结合胆碱乙酰转移酶（ChAT）之间的关系。对大鼠和人类大脑进行不同的增溶处理后，在可溶性和膜部分均获得了ChAT。向膜部分添加1%的 Triton X-100 导致 ChAT 释放。同时还观察到乳酸脱氢酶有类似的释放。通过免疫亲和层析有效地纯化了 Triton 从大鼠和人类大脑中释放的 ChAT 以及可溶性 ChAT。大鼠和人类大脑两种形式的 ChAT 均观察到单一分子量为68,000。两种形式的人类大脑 ChAT 产生的表位图谱相同。得出的结论是，Triton 释放的 ChAT 与可溶性 ChAT 相同，只是代表被包埋的可溶性 ChAT。
