人类疱疹病毒 6A U23 基因的特征。

Characterization of the human herpesvirus 6A U23 gene.

机构信息

Division of Clinical Virology, Kobe University Graduate School of Medicine, 7-5-1, Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan.

出版信息

Virology. 2014 Feb;450-451:98-105. doi: 10.1016/j.virol.2013.12.004. Epub 2013 Dec 22.

DOI:10.1016/j.virol.2013.12.004

PMID:24503071

Abstract

Human herpesvirus 6 (HHV-6), which replicates abundantly in T cells, belongs to the Roseolovirus genus within the betaherpesvirus subfamily. Members of the Roseolovirus genus encode seven unique genes, U20, U21, U23, U24, U24A, U26, and U100. The present study focused on one of these, U23, by analyzing the characteristics of its gene product in HHV-6A-infected cells. The results indicated that the U23 protein was expressed at the late phase of infection as a glycoprotein, but was not incorporated into virions, and mostly stayed within the trans Golgi network (TGN) in HHV-6A-infected cells. Furthermore, analysis using a U23-defective mutant virus showed that the gene is nonessential for viral replication in vitro.

摘要

人类疱疹病毒 6 型（HHV-6）在 T 细胞中大量复制，属于β疱疹病毒亚科中的玫瑰疹病毒属。玫瑰疹病毒属的成员编码七个独特的基因，U20、U21、U23、U24、U24A、U26 和 U100。本研究通过分析 HHV-6A 感染细胞中 U23 基因产物的特征，重点研究了其中一个基因 U23。结果表明，U23 蛋白在感染后期作为糖蛋白表达，但不被整合到病毒粒子中，并且主要存在于 HHV-6A 感染细胞的转高尔基网络（TGN）中。此外，使用 U23 缺失突变病毒进行的分析表明，该基因对于病毒在体外的复制并非必需。