Doebler J A, Markesbery W R, Anthony A, Scheff S W, Rhoads R E
Department of Biochemistry, University of Kentucky, Lexington 40536.
Acta Neuropathol. 1988;75(3):272-6. doi: 10.1007/BF00690535.
A new double-staining procedure, in which the techniques of immunocytochemistry of glial fibrillary acidic protein (GFAP) and quantitative microdensitometry of azure B-RNA were combined, was used to study nucleic acid alterations in fibrous astrocytes in Alzheimer's disease (AD). RNA contents of GFAP-positive cells of the hippocampal endplate (Rose's H3-H5 fields) and the dentate gyrus molecular layer were determined in ten autopsy-proven AD patients (ages 51-88) and ten age-matched, non-demented controls. In addition, RNA contents of pyramidal neurons of the endplate were examined. While there were no differences in RNA contents of astrocytes of either region between AD patients and controls, neuronal RNA was markedly depleted. These data suggest that astrocytes maintain protein synthetic capabilities in AD and that RNA loss is limited to the neuronal compartment.
一种新的双重染色程序被用于研究阿尔茨海默病（AD）中纤维性星形胶质细胞的核酸改变，该程序将胶质纤维酸性蛋白（GFAP）的免疫细胞化学技术与天青B - RNA的定量显微密度测定法相结合。在10例经尸检证实的AD患者（年龄51 - 88岁）和10例年龄匹配的非痴呆对照中，测定了海马终板（罗斯H3 - H5区）和齿状回分子层中GFAP阳性细胞的RNA含量。此外，还检查了终板锥体细胞的RNA含量。虽然AD患者和对照在这两个区域的星形胶质细胞RNA含量上没有差异，但神经元RNA明显减少。这些数据表明，在AD中星形胶质细胞维持着蛋白质合成能力，且RNA的丢失仅限于神经元部分。
