Programa de Pós-Graduação em Nutrição-Universidade Federal de Pernambuco (UFPE), Rua Nelson Chaves, s/n, Cidade Universitária, CEP 50670-901, Recife, Pernambuco, Brazil.
Appl Biochem Biotechnol. 2014 Mar;172(6):3234-52. doi: 10.1007/s12010-014-0761-1. Epub 2014 Feb 7.
Biosurfactants are of considerable interest due to their biodegradability, low degree of toxicity, and diverse applications. However, the high production costs involved in the acquisition of biosurfactants underscore the need for optimization of the production process to enable viable application on an industrial scale. The aims of the present study were to select a species of Candida that produces a biosurfactant with the greatest emulsifying potential and to investigate the influence of components of the production medium and cultivation conditions. Candida utilis achieved the lowest surface tension (35.53 mN/m), best emulsification index (73%), and highest yield (12.52 g/l) in a medium containing waste canola frying oil as the carbon source and ammonium nitrate as the nitrogen source. The best combination of medium components and cultivation conditions was 6% (w/v) glucose, 6% (w/v) waste canola frying oil, 0.2% (w/v) ammonium nitrate, 0.3% (w/v) yeast extract, 150 rpm, 1% inoculum (w/v), and 88 h of fermentation. The greatest biosurfactant production and the lowest surface tension were achieved in the first 24 h of production, and the maximum biomass production was recorded at 72 h. The biosurfactant produced from C. utilis under the conditions investigated in the present study has a potential to be a bioemulsifier for application in the food industry.
生物表面活性剂由于其可生物降解性、低毒性和多种应用而备受关注。然而，生物表面活性剂的获取成本高昂，这突显了需要优化生产工艺，以便在工业规模上可行地应用。本研究的目的是选择一种能产生具有最大乳化潜力的生物表面活性剂的 Candida 物种，并研究生产培养基成分和培养条件的影响。在含有废油菜籽油作为碳源和硝酸铵作为氮源的培养基中，Candida utilis 实现了最低的表面张力（35.53 mN/m）、最佳的乳化指数（73%）和最高的产率（12.52 g/l）。培养基成分和培养条件的最佳组合为 6%（w/v）葡萄糖、6%（w/v）废油菜籽油、0.2%（w/v）硝酸铵、0.3%（w/v）酵母提取物、150 rpm、1%接种物（w/v）和 88 h 的发酵。在本研究中调查的条件下，C. utilis 产生的生物表面活性剂的最大产量和最低表面张力出现在生产的前 24 小时内，最大生物量产量出现在 72 小时。在研究条件下产生的生物表面活性剂具有成为食品工业用生物乳化剂的潜力。
