Stockler-Pinto Milena Barcza, Mafra Denise, Moraes Cristiane, Lobo Julie, Boaventura Gilson Teles, Farage Najla Elias, Silva Wellington Seguins, Cozzolino Silvia Franciscato, Malm Olaf
Carlos Chagas Filho Biophysics Institute, Health Sciences Centre, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Rio de Janeiro, Brazil,
Biol Trace Elem Res. 2014 Apr;158(1):105-12. doi: 10.1007/s12011-014-9904-z. Epub 2014 Feb 8.
Cumulative evidence indicates that oxidative stress and inflammation frequently occurs in patients undergoing maintenance hemodialysis (HD) and as a result of overproduction of reactive oxygen species (ROS) and a decrease of antioxidant defenses such as selenium (Se). Previous studies in our laboratory showed that the supplementation of 1 unit of Brazil nut (the richest known food source of Se) a day during 3 months is effective to improve Se status and increase glutathione peroxidase (GPx) levels in HD patients. The aim of this study was to evaluate the effect of Brazil nut supplementation on oxidative stress and inflammation markers in HD patients. Forty HD patients from Rio de Janeiro, Brazil were studied. All patients received one nut per day for 3 months. The Se plasma levels and GPx, 8-isoprostane, 8-hydroxy-2-deoxyguanosine (8-OHdG), and cytokine (TNF-α and IL-6) levels and lipid profile were determined before and after 3 months of supplementation. The plasma Se and GPx activity increased, while cytokines, 8-OHdG, and 8-isoprostane plasma levels decreased significantly after 3 months supplementation. HDL-c levels increased and LDL-c levels decreased significantly. These data suggest that the consumption of only one Brazil nut per day during 3 months was effective to reduce the inflammation, oxidative stress markers, and the atherogenic risk, thereby increasing the antioxidant defenses in HD patients. Our results indicate that Brazil nut as Se source plays an important role as an anti-inflammatory and antioxidant agent in HD patients.
越来越多的证据表明，维持性血液透析（HD）患者经常出现氧化应激和炎症，这是由于活性氧（ROS）产生过多以及抗氧化防御能力下降，如硒（Se）减少所致。我们实验室之前的研究表明，在3个月内每天补充1单位巴西坚果（已知最丰富的硒食物来源）可有效改善HD患者的硒状态并提高谷胱甘肽过氧化物酶（GPx）水平。本研究的目的是评估补充巴西坚果对HD患者氧化应激和炎症标志物的影响。对来自巴西里约热内卢的40名HD患者进行了研究。所有患者每天食用一颗坚果，持续3个月。在补充3个月前后测定血浆硒水平、GPx、8-异前列腺素、8-羟基-2-脱氧鸟苷（8-OHdG）、细胞因子（TNF-α和IL-6）水平以及血脂谱。补充3个月后，血浆硒和GPx活性增加，而细胞因子、8-OHdG和8-异前列腺素血浆水平显著降低。高密度脂蛋白胆固醇（HDL-c）水平升高，低密度脂蛋白胆固醇（LDL-c）水平显著降低。这些数据表明，在3个月内每天仅食用一颗巴西坚果可有效减轻炎症、氧化应激标志物和动脉粥样硬化风险，从而增强HD患者的抗氧化防御能力。我们的结果表明，巴西坚果作为硒源在HD患者中作为抗炎和抗氧化剂发挥着重要作用。
