Department of Botany, University of Michigan, Ann Arbor.
Planta. 1969 Sep;87(3):217-26. doi: 10.1007/BF00389366.
Inhibitors of protein and RNA synthesis (cycloheximide, puromycin, chloramphenicol, and actinomycin D), as well as Co(++), induce opening of the hypocotyl hook of bean seedlings during the early stage of the opening period both in the darkness and red light. The response is transitory, however, complete straightening of a hook can not be achieved in the presence of these agents. These agents abolish the response of hooks to red illumination. They also block the suppression of hook opening caused by IAA and ethylene. The response and sensitivity to GA are not affected by the inhibitors. Inhibitors of DNA synthesis (FUDR and mitomycin C) have no effect on hook opening. It appears that in this growth response RNA and protein synthesis are more immediately involved in ethylene action than they are in the cell elongation process or the action of GA thereon.The results indicate that phytochrome does not induce hook opening simply by activating genes whose products directly promote growth. It is suggested that the regulation of ethylene formation by light and auxins may be exerted by way of influences on tissue levels of phenolic inhibitors of ethylene biosynthesis.
蛋白质和 RNA 合成抑制剂（环己亚胺、嘌呤霉素、氯霉素和放线菌素 D）以及 Co(++)，在暗中和红光下诱导豆苗下胚轴弯钩在开口期的早期打开。然而，这种反应是短暂的，在这些试剂存在的情况下，弯钩不能完全变直。这些试剂消除了弯钩对红光照射的反应。它们还阻止了 IAA 和乙烯引起的弯钩开放的抑制。DNA 合成抑制剂（FUDR 和丝裂霉素 C）对弯钩的打开没有影响。看来，在这种生长反应中，RNA 和蛋白质的合成比细胞伸长过程或 GA 对其的作用更直接地参与到乙烯作用中。结果表明，光敏色素并不是通过激活那些直接促进生长的基因来诱导弯钩打开的。有人认为，光和生长素对乙烯形成的调节可能是通过对组织水平的酚类乙烯生物合成抑制剂的影响来实现的。
