Botany Department, The Queen's University, Belfast.
Planta. 1966 Mar;69(1):70-8. doi: 10.1007/BF00380212.
Actinomycin-D inhibits phytochrome-mediated responses of etiolated plants. In the unrolling response of a barley first leaf the inhibition by act. D is greater when the antibiotic is applied 80 minutes after irradiation; earlier or later applications are less inhibitory. Inhibition is relieved by deoxyguanosine applied before or after act. D. Similar effects are found with the plumular hooks of peas and beans. These results suggest that phytochrome-mediated responses involve RNA production on a DNA template. The location of phytochrome in the cell is discussed in relation to its possible association with DNA, especially that of the plastids and mitochondria. Phytochrome may thus act as a repressor of gene sequences involved (for instance) in the removal of etiolation symptoms, red light (660 nm) causing its dissociation from DNA. Far-red radiation may reverse the effects by causing re-association of phytochrome.
放线菌素 D 抑制黄化植物的光敏素介导的反应。在大麦第一片叶子的展开反应中，抗生素在照射后 80 分钟应用时的抑制作用更大；更早或更晚的应用则抑制作用较小。脱氧鸟苷在放线菌素 D 之前或之后应用可缓解抑制作用。豌豆和菜豆的幼叶钩也有类似的效果。这些结果表明，光敏素介导的反应涉及 DNA 模板上的 RNA 产生。根据其与 DNA 的可能关联，特别是与质体和线粒体的关联，讨论了细胞中光敏素的位置。因此，光敏素可能作为涉及（例如）去除黄化症状的基因序列的阻遏物起作用，红光（660nm）导致其从 DNA 上解离。远红光可能通过引起光敏素的重新结合来逆转这种作用。
