Mittal K R, Higgins R, Larivière S
Département de Pathologie et de Microbiologie, Faculté de Médecine Vétérinaire, Université de Montréal, St-Hyacinthe, Québec, Canada.
Am J Vet Res. 1988 Feb;49(2):152-5.
Serotype-3 strains of Actinobacillus (Haemophilus) pleuropneumoniae possess epitopes shared with almost all other Actinobacillus serotypes. Common epitopes detected in particulate antigens were heat-labile and heat-stable and were of minor nature. Additional cross-reactive epitopes were detected in soluble and particulate antigens prepared from strains of serotypes 3, 6, and 8. Cross-reactions occurring between serotype-3 antigens and those of other serotypes were of 2 types: one associated mainly with 2-mercaptoethanol (2-ME)-sensitive IgM antibodies and the other associated mainly with 2-ME-resistant IgG antibodies. The cross-reactivity between serotypes 3, 6, and 8 was associated mainly with IgG antibodies, as shown by the results of 2-ME tube agglutination, 2-ME-indirect hemagglutination, and coagglutination tests. None of the tests was entirely satisfactory for serotyping serotype-3 isolates, mainly as a result of overlapping of type-specific antigenic determinants of serotypes 3, 6, and 8 in different combinations in the same strain. A combination of tests, using particulate and soluble antigens, may be necessary for typing of serotype-3 isolates.
胸膜肺炎放线杆菌（嗜血杆菌）血清型3菌株具有与几乎所有其他放线杆菌血清型共有的表位。在颗粒性抗原中检测到的共同表位有热不稳定型和热稳定型，且性质次要。在由血清型3、6和8菌株制备的可溶性和颗粒性抗原中检测到了额外的交叉反应性表位。血清型3抗原与其他血清型抗原之间发生的交叉反应有两种类型：一种主要与对2-巯基乙醇（2-ME）敏感的IgM抗体相关，另一种主要与对2-ME有抗性的IgG抗体相关。如2-ME试管凝集试验、2-ME间接血凝试验和协同凝集试验结果所示，血清型3、6和8之间的交叉反应主要与IgG抗体相关。对于血清型3分离株的血清分型，没有一种试验是完全令人满意的，主要是因为在同一菌株中血清型3、6和8的型特异性抗原决定簇以不同组合重叠。对于血清型3分离株的分型，可能需要使用颗粒性和可溶性抗原进行联合试验。
