Udensi O U, Ontui V
Department of Genetics and Biotechnology, University of Calabar, PMB 1115, Calabar, Nigeria.
Pak J Biol Sci. 2013 Jul 1;16(13):630-5. doi: 10.3923/pjbs.2013.630.635.
The need to optimize flow cytometric analysis for the determination of ploidy level is a worthwhile venture to precisely know at what concentration of a mutagen and at what time of exposure polyploidy could be induced. Flow cytometry was used to determine the polyploidy inducing-capacity of colchicine in pigeon pea (Cajanus cajan (L.) Mill sp). Seeds of pigeon pea were soaked in three different concentrations of colchicine-5 mg, 10 and 15 mg L(-1) for 24, 48 and 72 h, respectively, while the control group was soaked in water. Treated seeds and those from the control were planted in a greenhouse using a Completely Randomized Design (CRD). Results show that colchicine induced tetraploids (4n) and mixoploids (2n+ 4n) as the concentration of colchicine increased and soaking duration. Days to seedling emergence increased as concentration of colchicine and duration of soaking increased while germination rate decreased proportionately with the increase in colchicine concentration and soaking duration but did not significantly affect percentage seedling survival. Explicitly, colchicine has the capacity of inducing polyploidy; especially tetraploids on the seeds of pigeon pea, which obviously could be harnessed for further breeding and improvement of the pigeon pea.
优化流式细胞术分析以确定倍性水平的必要性是一项有价值的工作，有助于精确了解在何种诱变剂浓度和暴露时间下可诱导多倍体的产生。采用流式细胞术测定秋水仙碱对木豆（Cajanus cajan (L.) Mill sp）的多倍体诱导能力。将木豆种子分别浸泡在三种不同浓度（5毫克、10毫克和15毫克/升）的秋水仙碱溶液中24小时、48小时和72小时，对照组种子浸泡在水中。处理后的种子和对照组种子采用完全随机设计（CRD）种植在温室中。结果表明，随着秋水仙碱浓度和浸泡时间的增加，秋水仙碱诱导出四倍体（4n）和混倍体（2n + 4n）。随着秋水仙碱浓度和浸泡时间的增加，出苗天数增加，而发芽率随秋水仙碱浓度和浸泡时间的增加成比例下降，但对幼苗成活率没有显著影响。明确地说，秋水仙碱具有诱导多倍体的能力；特别是对木豆种子诱导出四倍体，这显然可用于木豆的进一步育种和改良。
