Tammu Reisky Megawati, Nuringtyas Tri Rini, Daryono Budi Setiadi
Biology Education Department - Faculty of Education, Universitas Pelita Harapan, Jl. M. H. Thamrin, Lippo Karawaci, Tangerang, Banten, 15811, Indonesia.
Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan, Sekip Utara, Sleman, 55281, DI Yogyakarta, Indonesia.
J Genet Eng Biotechnol. 2021 Feb 12;19(1):31. doi: 10.1186/s43141-021-00131-4.
Productivity and quality of crops can be increased through polyploidy plants induced by colchicine. The use of colchicine has never been applied to Katokkon pepper, a local red pepper variety from North Toraja Indonesia. This pepper is characterized by its unique shape like the small-bell pod type of paprika and its strong spicy taste. Therefore, this study aimed to determine the effects of colchicine treatment on the ploidy level and morphological characters of Katokkon pepper.
Flow cytometer analysis showed that all colchicine concentration treatments ranging from 0.025 to 0.1% with 24 h immersion time generated two plant groups based on their ploidy level; 50% of the total treatment plants was diploid while the rest plants were mixoploid. All colchicine treatment plants were significantly different in their width of stomata guard cells from the control plant. The effect of colchicine was also significantly on the plant height, thickness of fruit flesh, and the number of fruits per plant.
The results of this study showed that colchicine treatment had a significant effect on the ploidy level and several morphological characters of the Katokkon pepper. The colchicine treatment increased the number of fruits per plant and thicker flesh of fruits but reduced the size and weight of Katokkon pepper. Our findings provide essential information to obtain tetraploid Katokkon plants through colchicine treatment in further research. This study benefits as a preliminary step for increasing the productivity and quality of the local red peppers in Indonesia.
通过秋水仙碱诱导的多倍体植物可以提高作物的产量和品质。秋水仙碱从未被应用于卡托克孔辣椒，这是一种来自印度尼西亚北托拉雅的当地红辣椒品种。这种辣椒的特点是其独特的形状，类似于小铃铛荚类型的甜椒，并且辣味强烈。因此，本研究旨在确定秋水仙碱处理对卡托克孔辣椒倍性水平和形态特征的影响。
流式细胞仪分析表明，所有秋水仙碱浓度处理（0.025%至0.1%），浸泡时间为24小时，根据倍性水平产生了两个植物组；处理植物总数的50%是二倍体，其余植物是混倍体。所有秋水仙碱处理的植物与对照植物相比，其气孔保卫细胞宽度存在显著差异。秋水仙碱对株高、果肉厚度和单株果实数量也有显著影响。
本研究结果表明，秋水仙碱处理对卡托克孔辣椒的倍性水平和几个形态特征有显著影响。秋水仙碱处理增加了单株果实数量和果实果肉厚度，但减小了卡托克孔辣椒的大小和重量。我们的研究结果为在进一步研究中通过秋水仙碱处理获得四倍体卡托克孔植物提供了重要信息。本研究作为提高印度尼西亚当地红辣椒产量和品质的初步步骤具有重要意义。
