Wada T, Uede T, Ishii S, Matsuyama K, Yamawaki S, Kikuchi K
Department of Pathology, Sapporo Medical College, S-1, Japan.
Cancer Res. 1988 Apr 15;48(8):2273-9.
Monoclonal antibodies (MoAbs) against human osteosarcoma cells were obtained by the fusion of NS/1 mouse myeloma cells with spleen cells from the human osteosarcoma cell line-immunized BALB/c mice. Two hybrid clones were established and designated as 2H10 and 2D3. Both MoAbs reacted strongly with all osteosarcoma tissues but not with other bone and soft tissue tumors such as chondrosarcoma, malignant fibrous histiocytoma, liposarcoma, leiomyosarcoma, and rhabdomyosarcoma. In addition, neither MoAb reacted with tumor cell lines and tissues obtained from other cancers. Immunohistochemical analysis demonstrated that 2H10 and 2D3 reacted with endothelial cells in sarcoma tissues, but not with those of other tumors and normal tissues. 2H10 also reacted with cells on the basal layer of epidermis of the skin. 2H10- and 2D3-defined antigen has an approximate molecular weight of 75,000 under nonreducing and reducing conditions, indicating that the antigen has a single chain structure and there is no intramolecular disulfide bond. 2H10- and 2D3-defined antigen has a pI value between 5.5 and 6.2. Sequential immunoprecipitation analysis clearly demonstrated that 2H10 and 2D3 recognized the same antigen molecule. However, further analysis suggested the possibility that 2H10 and 2D3 MoAbs recognized the different antigenic determinants on the same antigen molecule.
通过将 NS/1 小鼠骨髓瘤细胞与用人骨肉瘤细胞系免疫的 BALB/c 小鼠的脾细胞融合，获得了抗人骨肉瘤细胞的单克隆抗体（MoAbs）。建立了两个杂交克隆，分别命名为 2H10 和 2D3。两种 MoAbs 均与所有骨肉瘤组织强烈反应，但不与其他骨和软组织肿瘤反应，如软骨肉瘤、恶性纤维组织细胞瘤、脂肪肉瘤、平滑肌肉瘤和横纹肌肉瘤。此外，两种 MoAbs 均不与从其他癌症获得的肿瘤细胞系和组织反应。免疫组织化学分析表明，2H10 和 2D3 与肉瘤组织中的内皮细胞反应，但不与其他肿瘤和正常组织的内皮细胞反应。2H10 还与皮肤表皮基底层的细胞反应。在非还原和还原条件下，2H10 和 2D3 所定义的抗原的分子量约为 75,000，表明该抗原具有单链结构且不存在分子内二硫键。2H10 和 2D3 所定义的抗原的 pI 值在 5.5 至 6.2 之间。连续免疫沉淀分析清楚地表明，2H10 和 2D3 识别相同的抗原分子。然而，进一步分析表明，2H10 和 2D3 MoAbs 可能识别同一抗原分子上不同的抗原决定簇。
