通过与人类骨肉瘤相关抗原发生反应的单克隆抗体检测骨型碱性磷酸酶。
Detection of bone-type alkaline phosphatase by monoclonal antibodies reacting with human osteosarcoma-associated antigen.
作者信息
Nagoya S, Uede T, Wada T, Ishii S, Yamawaki S, Kikuchi K
机构信息
Department of Pathology, Sapporo Medical College.
出版信息
Jpn J Cancer Res. 1991 Jul;82(7):862-70. doi: 10.1111/j.1349-7006.1991.tb02714.x.
The antigen detected by monoclonal antibodies reacting with human osteosarcoma-associated antigen was shown to be a phosphatidyl-inositol (PI)-glycan-anchored protein, which can be released from the cell surface by PI-specific phospholipase C-treatment. The antigen detected by 2D3 and 2H10 antibodies exhibited alkaline phosphatase activity. Both antibodies strongly reacted with bone-type alkaline phosphatase. However, importantly, immunohistochemical analysis demonstrated that 2D3 and 2H10 did not react with alkaline phosphatase present in kidney or liver. In addition, neither placental nor intestinal alkaline phosphatase was recognized by 2D3 and 2H10 antibodies. These results indicated that two monoclonal antibodies, 2D3 and 2H10, are highly specific for bone-type alkaline phosphatase and can distinguish bone alkaline phosphatase from liver alkaline phosphatase in spite of the fact that liver and bone alkaline phosphatase are encoded by the same gene.
与人类骨肉瘤相关抗原发生反应的单克隆抗体所检测到的抗原被证明是一种磷脂酰肌醇（PI）-聚糖锚定蛋白，该蛋白可通过PI特异性磷脂酶C处理从细胞表面释放。2D3和2H10抗体所检测到的抗原表现出碱性磷酸酶活性。两种抗体均与骨型碱性磷酸酶发生强烈反应。然而，重要的是，免疫组织化学分析表明，2D3和2H10与肾脏或肝脏中存在的碱性磷酸酶不发生反应。此外，2D3和2H10抗体也未识别胎盘碱性磷酸酶或肠道碱性磷酸酶。这些结果表明，两种单克隆抗体2D3和2H10对骨型碱性磷酸酶具有高度特异性，并且尽管肝脏碱性磷酸酶和骨碱性磷酸酶由同一基因编码，但它们仍能将骨碱性磷酸酶与肝脏碱性磷酸酶区分开来。
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