[白细胞介素-10启动子多态性及血浆水平与声带白斑易感性的关联]

[Association of IL-10 promoter polymorphism and plasma levels with susceptibility to vocal cords leukoplakia].

作者信息

Zhou Jian, Tao Lei, Zhang Duo, Chen Bin, Tang Wei-jing, Lu Li-ming

机构信息

Department of Otorhinolaryngology Head and Neck Surgery, Eye, Ear, Nose and Throat Hospital of Fudan University, Shanghai 200031, China.

Shanghai Institute of Immunology, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China. Email:

出版信息

Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2013 Dec;48(12):1017-21.

PMID:24507002

Abstract

OBJECTIVE

To investigated the association between interleukin-10(IL-10)-1082/-819/-592 promoter polymorphism, plasma IL-10 levels and risk of vocal leukoplakia.

METHODS

A case-control study of 61 patients with vocal cords leukoplakia and 119 healthy subjects were performed. Genotypes for the IL-10 gene (IL-10 -1082 G/A, -819 C/T and -592 C/A) were determined using pyrosequencing and plasma IL-10 levels were analyzed by ELISA.

RESULTS

Smoking and alcohol consumption increased the risk of vocal leukoplakia (OR = 4.73, 95%CI:2.4-9.1;OR = 5.70, 95%CI:2.9-11.2, P < 0.01) . Patients with vocal cord leukoplakia had significantly higher frequencies of AC at -592 and -819 (OR = 1.93, 95%CI:0.97-3.81, P = 0.05) and AG at -1082(OR = 2.14, 95%CI:0.87-5.27, P = 0.092) than control. Vocal leukoplakia patients had higher concentration of plasma IL-10 (21.6 ± 0.5) pg/ml (X(-) ± s) than controls (19.0 ± 1.1)pg/ml(t = 2.08, P = 0.043) and the haplotype containing the G allele was associated with higher plasma IL-10 concentrations (24.3 ± 5.7) pg/ml compared with the ATA haplotype(19.9 ± 4.7) pg/ml (t = -2.64, P = 0.008).

CONCLUSIONS

IL-10-1082/-819/-592 promoter polymorphism and high concentration of plasma IL-10 are associated with vocal cord leukoplakia, and the concentration of plasma IL-10 is associated with the genotypes of IL-10 promoter polymorphism.

摘要

目的

研究白细胞介素-10（IL-10）-1082/-819/-592启动子多态性、血浆IL-10水平与声带白斑发病风险之间的关联。

方法

对61例声带白斑患者和119例健康受试者进行病例对照研究。采用焦磷酸测序法测定IL-10基因（IL-10 -1082 G/A、-819 C/T和-592 C/A）的基因型，并用酶联免疫吸附测定法分析血浆IL-10水平。

结果

吸烟和饮酒会增加声带白斑的发病风险（比值比=4.73，95%置信区间：2.4-9.1；比值比=5.70，95%置信区间：2.9-11.2，P<0.01）。声带白斑患者中，-592和-819位点AC基因型（比值比=1.93，95%置信区间：0.97-3.81，P=0.05）以及-1082位点AG基因型（比值比=2.14，95%置信区间：0.87-5.27，P=0.092）的频率显著高于对照组。声带白斑患者的血浆IL-10浓度（21.6±0.5）pg/ml（均数±标准差）高于对照组（19.0±1.1）pg/ml（t=2.08，P=0.043），并且含有G等位基因的单倍型与较高的血浆IL-10浓度（24.3±5.7）pg/ml相关，而ATA单倍型为（19.9±4.7）pg/ml（t=-2.64，P=0.008）。