Weetman A P, Tse C K, Randall W R, Tsim K W, Barnard E A
Department of Medicine, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK.
Clin Exp Immunol. 1988 Jan;71(1):96-9.
It has been suggested that anti-thyroglobulin antibodies cross-react with acetylcholinesterase (AChE) and that this may explain the pathogenesis of Graves' ophthalmopathy. We have tested this hypothesis using an ELISA. Antibodies to human red blood cell AChE were found in 21% of 47 patients with thyroid autoimmunity. However antibodies to AChE were also detected in one of 25 normal subjects and two of 16 patients with non-organ specific autoimmunity. The anti-AChE antibodies showed no correlation with anti-thyroglobulin antibody levels and they were not associated with the presence of severe ophthalmopathy. Inhibition studies suggested only limited cross reactivity, if any, between anti-Tg and anti-AChE antibodies. Immunoblotting demonstrated antibody binding to at least four human AChE determinants at 130, 55, 32 and 22 kD. Our results demonstrate quite frequent anti-AChE reactivity in sera but no relationship with the development of orbital pathology.
有人提出抗甲状腺球蛋白抗体与乙酰胆碱酯酶（AChE）发生交叉反应，这可能解释了格雷夫斯眼病的发病机制。我们使用酶联免疫吸附测定（ELISA）对这一假说进行了检验。在47例甲状腺自身免疫患者中，21%的患者体内发现了抗人红细胞AChE抗体。然而，在25名正常受试者中的1人以及16例非器官特异性自身免疫患者中的2人体内也检测到了抗AChE抗体。抗AChE抗体与抗甲状腺球蛋白抗体水平无相关性，且与严重眼病的存在无关。抑制研究表明，抗Tg和抗AChE抗体之间即使存在交叉反应，也非常有限。免疫印迹显示抗体与130、55、32和22 kD的至少四种人AChE决定簇结合。我们的结果表明血清中抗AChE反应相当常见，但与眼眶病变的发展无关。
